IM-54 |
Catalog No.GC41295
One-Click Copy Product Info
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IM-54 is a cell-permeable oxidative stress-induced necrosis inhibitor.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 861891-50-1
Sample solution is provided at 25 µL, 10mM.
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IM-54 is a cell-permeable oxidative stress-induced necrosis inhibitor. IM-54 inhibits H2O2- and TBHP-induced mitochondrial-associated necrosis signals to reduce oxidative stress-induced cell death. IM-54 can be used for research on oxidative damage, ischemia-reperfusion injury, myocardial infarction, and stroke-related cell necrosis[1-4].
3µM IM-54 was used to treat nitidine chloride-induced H1688 and A549 cells for 48h, and did not protect against cell death[5]. 210nM IM-54 was used to treat H2O2-induced HL-60 cells for 0.5h, inhibited necrotic cell death and reduced lactate dehydrogenase release[6]. 3µM IM-54 was used to treat 4-hydroxychalcone-induced SK-N-BE(2) and IMR-32 cells for 1h, prevented cell death and decreased reactive oxygen species[7].
References:[1] Uemichi Y, Yasuda S, Mabuchi M, et al. Effects of Azelastine on Glioblastoma Cells. Anticancer Res. 2025 Jul;45(7):2763-2771.
[2] Sodeoka M, Dodo K. Development of selective inhibitors of necrosis. Chem Rec. 2010 Oct;10(5):308-14.
[3] Tomasek T, Ware LB, Bastarache JA, et al. Cell-free hemoglobin-mediated human lung microvascular endothelial barrier dysfunction is not mediated by cell death. Biochem Biophys Res Commun. 2021 Jun 4;556:199-206.
[4] Zeng F, Sherry J P, Bols N C. Evaluating the toxic potential of benzothiazoles with the rainbow trout cell lines, RTgill-W1 and RTL-W1. Chemosphere. 2016 Aug;155:308-318.
[5] Yu F, Tan W, Chen Z, et al. Nitidine chloride induces caspase 3/GSDME-dependent pyroptosis by inhibting PI3K/Akt pathway in lung cancer. Chin Med. 2022 Sep 29;17(1):115.
[6] Dodo K, Katoh M, Shimizu T, et al. Structure-Activity Relationship Study of 3-Amino-2-indolyllactam Derivatives: Development of Inhibitors of Oxidative Stress-Induced Necrosis. Chem Pharm Bull. 2016 Jul;64(7):886-898.
[7] Alshangiti A, Tuboly E, Hegarty S, et al. 4-Hydroxychalcone Induces Cell Death via Oxidative Stress in MYCN-Amplified Human Neuroblastoma Cells. Oxidative Medicine and Cellular Longevity. 2019 Dec 5;2019:1670759.
| Cell experiment [1]: | |
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Cell lines |
HL-60 cells (human promyelocytic leukemia cell line) |
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Preparation Method |
HL-60 cells were maintained in RPMI 1640 medium supplemented with 100U/mL penicillin, 100μg/mL streptomycin, 5% heat-inactivated fetal bovine serum (FBS) at 37°C, 5% CO2. HL-60 cells were pretreated with IM-54 for 0.5h, then treated with hydrogen peroxide (100μM) for 3h. After treatment, lactate dehydrogenase (LDH) release was quantified. |
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Reaction Conditions |
210nM; 0.5h pretreatment |
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Applications |
IM-54 inhibited hydrogen peroxide-induced necrotic cell death in HL-60 cells. |
| References: [1] Dodo K, Hayamizu K, Shimizu T, et al. Structure-Activity Relationship Study of 3-Amino-2-indolyllactam Derivatives: Development of Inhibitors of Oxidative Stress-Induced Necrosis. Chem Pharm Bull. 2016 Jul;64(7):886-898. | |
| Cas No. | 861891-50-1 | SDF | |
| Canonical SMILES | CCCCCNC1=C(C(=O)N(C)C1=O)c1cn(C)c2ccccc12 | ||
| Formula | C19H23N3O2 | M.Wt | 325.4 |
| Solubility | 1 mg/ml in DMSO; 20 mg/ml in DMF | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0731 mL | 15.3657 mL | 30.7314 mL |
| 5 mM | 614.6 μL | 3.0731 mL | 6.1463 mL |
| 10 mM | 307.3 μL | 1.5366 mL | 3.0731 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 7 reference(s) in Google Scholar.)