Liperfluo |
Catalog No.GC26099
One-Click Copy Product Info
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Liperfluo is a marker of iron death and a useful fluorescent probe, which can be used to study the role of lipid peroxidation in various cell pathophysiology.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1448846-35-2
Sample solution is provided at 25 µL, 10mM.
Liperfluo is a marker of iron death and a useful fluorescent probe, which can be used to study the role of lipid peroxidation in various cell pathophysiology. Liperfluo reduces lipid hydroperoxide to lipid alcohol, which can be used to image lipid hydroperoxide in living cells.
This plan only provides a guide, please modify it to meet your specific needs.
1. Prepare dyeing solution
(1) Dye stock solution: Centrifuge the tube before use to concentrate the powder at the bottom of the tube. Suggest dissolving the powder in DMSO and preparing a stock solution with a concentration of 10mM. The pre-configured stock solution should be packaged and stored in a sealed manner at -20℃ or -80℃, away from moisture and light.
(2) Dye working solution: Dilute the stock solution with a suitable buffer solution (such as HBSS) and prepare Liperfluo working solution with a concentration of 10μM. Please adjust and optimize the concentration of the working solution according to the actual situation, and prepare it as needed.
2. Cell suspension staining (Taking 6-well plate as an example)
(1) Suspension cells were centrifuged at 1000g for 3-5 minutes. Discard the supernatant and clean twice with HBSS for 5 minutes each time.
(2) Wash the adherent cells twice with HBSS, add Trypsin to digest the cells, centrifuge at 1000g for 3-5 minutes after digestion, and discard the supernatant.
(3) Add 1mL of dye working solution to resuspend the cells and incubate at 37℃ in the dark for 30 minutes. The optimal incubation time for different cells varies, please adjust according to the actual situation.
(4) After incubation, centrifuge at 1000g for 5 minutes to remove the supernatant, and wash 2-3 times with HBSS for 5 minutes each time.
(5) Use HBSS to resuspend cells and observe them using fluorescence microscopy or flow cytometry.
3. Cell adhesion staining
(1) Cultivate adherent cells on sterile coverslips.
(2) Remove the cover glass from the culture medium, aspirate excess culture medium, and place the cover glass in a damp environment.
(3) Add 100μL of dye working solution from one corner of the cover glass, gently shake to evenly cover all cells with dye, and incubate at 37℃ in the dark for 30 minutes. The optimal culture time for different cells varies, please adjust according to the actual situation.
(4) Discard the dye working solution and clean the cover glass 2-3 times with HBSS, each time for 5 minutes.
4. Observe using a fluorescence microscope or flow cytometer. The maximum excitation/emission wavelength of Liperfluo is 524/535nm.
Precautions:
① Fluorescent dyes all have quenching problems. Please try to avoid light to slow down fluorescence quenching;
② For your safety and health, please wear a lab coat and disposable gloves.
| Cas No. | 1448846-35-2 | SDF | |
| Formula | C51H41N2O8P | M.Wt | 840.85 |
| Solubility | DMSO : 8.5 mg/mL (10.11 mM; Need ultrasonic and warming) | Storage | 4°C, sealed storage, away from moisture and light. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.1893 mL | 5.9464 mL | 11.8927 mL |
| 5 mM | 237.9 μL | 1.1893 mL | 2.3785 mL |
| 10 mM | 118.9 μL | 594.6 μL | 1.1893 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)