MEGA-10 (Synonyms: N-decanoyl-N-Methylglucamine) |
Catalog No.GC44151
One-Click Copy Product Info
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MEGA-10 is a nonionic detergent that can be used to solubilize membrane proteins.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 85261-20-7
Sample solution is provided at 25 µL, 10mM.
MEGA-10 is a nonionic detergent that can be used to solubilize membrane proteins. [1] It has a critical micelle concentration (CMC) of 4.88 mM under no salt conditions, which decreases in the presence of sodium chloride. [2] MEGA-10 has been used to reconstitute amino acid transporters from rat liver plasma membrane vesicles into artificial phospholipid membranes.[3] It has also been used to solubilize the melibiose transport carrier from E. coli membranes and reconstitute it into liposomes.[4]
Reference:
[1]. Hildreth, J.E.K. N-D-Gluco-N-methylalkanamide compounds, a new class of non-ionic detergents for membrane biochemistry. Biochem. J. 207(2), 363-366 (1982).
[2]. Molina-Bolívar, J.A., Hierrezuelo, J.M., and Carnero Ruiz, C. Self-assembly, hydration, and structures in N-decanoyl-N-methylglucamide aqueous solutions: Effect of salt addition and temperature. J. Colloid. Interface Sci. 313(2), 656-664 (2007).
[3]. Quesada, A.R., and McGivan, J.D. A rapid method for the functional reconstitution of amino acid transport systems from rat liver plasma membranes. Partial purification of System A. Biochem J. 255(3), 963-969 (1988).
[4]. Hanatani, M., Nishifuji, K., Futai, M., et al. Solubilization and reconstitution of membrane proteins of Escherichia coli using alkanoyl-N-methylglucamides. J. Biochem. 95(5), 1349-1353 (1984).
| Cas No. | 85261-20-7 | SDF | |
| Synonyms | N-decanoyl-N-Methylglucamine | ||
| Chemical Name | 1-deoxy-1-[methyl(1-oxodecyl)amino]-D-glucitol | ||
| Canonical SMILES | OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)CN(C)C(CCCCCCCCC)=O | ||
| Formula | C17H35NO6 | M.Wt | 349.5 |
| Solubility | 1mg/mL in ethanol, 30mg/mL in DMSO or in DMF | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8612 mL | 14.3062 mL | 28.6123 mL |
| 5 mM | 572.2 μL | 2.8612 mL | 5.7225 mL |
| 10 mM | 286.1 μL | 1.4306 mL | 2.8612 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 19 reference(s) in Google Scholar.)