NG,NG-dimethyl-L-Arginine (hydrochloride) (Synonyms: ADMA) |
| Catalog No.GC17237 |
endogenous nitric oxide synthase inhibitor
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 220805-22-1
Sample solution is provided at 25 µL, 10mM.
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NG,NG-dimethyl-L-Arginine is an endogenous nitric oxide synthase inhibitor.
Nitric oxide (NO) that is synthesised from L-arginine contributes to the regulation of blood pressure and to host defence.
In vitro: NG,NG-dimethyl-L-Arginine (ADMA) was found to be able to inhibit macrophage and vascular NO synthase. When added to a cytosolic preparation of macrophage NO synthase, ADMA could cause dose-dependent inhibition of NO formation. In the presence of added arginine, ADMA could inhibited NO synthesis and the inhibitory effects of ADMA were prevented by addition of L-arginine so that the rate of NO synthesis did not significantly differ from control values [1].
In vivo: Animal study showed that the injection of 1-30 mg/kg ADMA into 3 anaesthetised guineapigs could cause dose-dependent increases in mean arterial blood pressure. Moreover, during continuous infusion of ADMA, which caused plasma ADMA concentrations to rise approximately 9-fold, the systolic blood pressure was raised by nearly 15 % [1].
Clinical trial: Local infusion of ADMA into the brachial artery of healthy volunteers caused a dose-dependent fall in forearm blood-flow. Infusion of ADMA for 5 min resulted in a fall in forearm blood-flow in 5 subjects, and the effects of this ADMA dose were significantly attenuated by infusion of L-arginine [1].
Reference:
[1] Vallance, P. ,Leone, A.,Calver, A., et al. Accumulation of an endogenous inhibitor of nitric oxide synthesis in chronic renal failure. Lancet 339, 572-575 (1992).
| Cas No. | 220805-22-1 | SDF | |
| Synonyms | ADMA | ||
| Chemical Name | N5-[(dimethylamino)iminomethyl]-L-ornithine, dihydrochloride | ||
| Canonical SMILES | OC(=O)[C@@H](N)CCCNC(=N)N(C)C | ||
| Formula | C8H18N4O2 • 2HCl | M.Wt | 275.2 |
| Solubility | DMF: 5 mg/ml; DMSO: 3 mg/ml; Ethanol: 3 mg/ml; PBS (pH 7.2): 20 mg/ml; Water: 20 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.6337 mL | 18.1686 mL | 36.3372 mL |
| 5 mM | 0.7267 mL | 3.6337 mL | 7.2674 mL |
| 10 mM | 0.3634 mL | 1.8169 mL | 3.6337 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 19 reference(s) in Google Scholar.)
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