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Phosphatase Inhibitor Cocktail II (100× in ddH₂O) Catalog No.GK10012

Phosphatase Inhibitor Cocktail II effectively inhibits acid and alkaline phosphatases as well as protein tyrosine phosphatases(PTPs).

Size Price Stock Qty
1mL
$22.50
In stock
1 mL × 10
$155.00
In stock
1 mL × 50
$647.00
In stock
1 mL × 100
$971.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Protocol

Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Background

Phosphatase Inhibitor Cocktail II effectively inhibits acid and alkaline phosphatases as well as protein tyrosine phosphatases(PTPs).

Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts, including samples containing detergents. Provided as 1 ml vial containing the following components: Imidazole (200 mM), Sodium Fluoride (100 mM), Sodium Molybdate (115 mM), Sodium Orthovanadate (100 mM), and Sodium Tartrate Dihydrate (400 mM).

Phosphatase Inhibitor Cocktail II has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in ddH2O.

Components

Ingredient M.W. 100x Conc. Target
Sodium Fluoride 42.0 100 mM Acid phosphatases
Sodium Orthovanadate 183.9 100 mM Alkaline phosphatases, PTPs, ATPases
Sodium Tartrate Dihydrate 230.1 400 mM Acid phosphatases
Sodium Molybdate 205.9 115 mM Acid and phosphoprotein phosphatases
Imidazole 68.1 200 mM Alkaline phosphatases