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Phosphatase Inhibitor Cocktail III (100× in DMSO) Catalog No.GK10013

Phosphatase Inhibitor Cocktail III effectively inhibits serine/threonine and alkaline phosphatases.

Size Price Stock Qty
1mL
$32.20
In stock
1 mL × 10
$231.00
In stock
1 mL × 50
$1,076.00
In stock
1 mL × 100
$1,618.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Protocol

Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Background

Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three phosphatase inhibitors for the inhibition of both serine/threonine and alkaline phosphatases. Each vial contains a 1 ml solution with the following components: 500 µM Cantharidin, 2.5 mM (-)-p-Bromotetramisole oxalate, and 1 µM Calyculin A form Discodermia calyx.

Phosphatase Inhibitor Cocktail III has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.

Components

Ingredient M.W. 100x Conc. Target
(-)-p-Bromolevamisole oxalate 373.2 2.5 mM Alkaline phosphatases
Cantharidin 196.2 500 µM protein phosphatase 2A(PP2A)
Calyculin A 1009.2 1 µM protein phosphatases 1 and 2A(PP1 and PP2A)