SYTO 9 (20x in DMSO) Nucleic Acid Dyes for PCR

SYTO9 Gren fluorescent Nucleic Acid Stain (SVTO9 Gren fluorescent Nucleic Acid Stain) is an excellent counterstain for nuclei and chromosomes and is highly permeable to cell membranes. SYTO 9 binds DNA (and RNA) with high affinity. Once bound, it shows a significantly enhanced fluorescence signal. It is excited by blue light (maximum emission wavelength 485nm) and emits green fluorescence (maximum excitation is 498nm (DNA) and 501nm (RNA) respectively). . Because SYTO 9 can penetrate well into prokaryotic and eukaryotic cell membranes, it is commonly used as a nuclear counterstain (especially bacterial cells). It is often used in combination with dead cell nuclear counterstains (such as propidium iodide PI). Live cell/dead bacterial staining.

What is more noteworthy is that SYTO 9, as a sensitive DNA-binding dye, exhibits many excellent characteristics in RT-PCR, including: producing highly reproducible DNA melting curves under a wide range of dye concentrations, extremely low PCR inhibition rate, high signal-to-noise ratio and high sensitivity make it an ideal SYBR Green I alternative dye for RT-PCR, DNA melting curve analysis, and loop-mediated isothermal amplification (LAMP) experiments [3- 5].
This product is 20×SYTO 9 nucleic acid dye dissolved in DMSO, reaching PCR level. When using it, you only need to add the amount according to the system to make the final concentration 1×.

Storage and transportation methods
Storage: Store at 2-8°C away from light, or at -20°C. Valid for 2 years.
Shipping: Ice pack shipping.