WY165 |
Catalog No.GC79008
One-Click Copy Product Info
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WY165 is a bifunctional molecule comprising TR79, an activator of the mitochondrial protease complex caseinolytic protease P ( ClpP ), linked to desthiobiotin.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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In Vitro, WY165 (10-10000 nM; 24 h) decreases mSA in a dose-dependent manner[1]. WY165's (1 μM; 24 h) mSA degradation activity is inhibited by excess desthiobiotin (1 mM), suggesting that the formation of the [mSA-WY165-ClpP] ternary complex is required[1]. WY165 (0.3-30 μM; 2-12 h) reduced mitochondrial mSA levels in HeLa and MCF7 cells transiently expressing mitochondrial cox8-mSA-FLAG in a dose- and time-dependent manner, with DC50 values for mSA of 4.8 μM and 0.96 μM, respectively[1]. WY165-induced (10 μM; 48 h) degradation of mSA depends on ClpP in HeLa cells[1]. WY165 (1-30 μM) decreases mSA-STMP1 in a dose-dependent manner in HeLa cells transiently expressing cox8-His6-mSA-STMP1-FLAG[1]. WY165 (1 μM) successfully restores the changes in mitochondrial morphology in HeLa cells expressing mSA-STMP1 and do not change mitochondrial morphology in HeLa cells not expressing mSA-STMP1[1].
References:
[1]. Yamada W, et al. Targeted protein degradation in the mitochondrial matrix and its application to chemical control of mitochondrial morphology. Chem Sci. 2024 Aug 28;15(36):14625–34.
| Cas No. | SDF | ||
| Formula | C46H61F3N8O9 | M.Wt | 927.02 |
| Solubility | DMSO: 60 mg/mL (64.72 mM; ultrasonic and warming and heat to 60°C) | Storage | Store at -20°C, sealed storage, away from moisture and light |
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.0787 mL | 5.3936 mL | 10.7873 mL |
| 5 mM | 215.7 μL | 1.0787 mL | 2.1575 mL |
| 10 mM | 107.9 μL | 539.4 μL | 1.0787 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)