AM 404 (Synonyms: 4HPA, N(4hydroxyphenyl)Arachidonoyl Amide) |
| Catalog No.GC12071 |
Anandamide transport inhibitor
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 198022-70-7
Sample solution is provided at 25 µL, 10mM.
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AM 404 is a selective inhibitor of anandamide transport [1]. Also, it is an agonist of CB1 cannabinoid receptor and potential vanilloid type 1 (TRPV1).
The endocannabinoid transporter (eCBT) is a transporter for the endocannabinoid. The blockade of anandamide transport has anti-nociceptive effects.
AM 404 is a selective anandamide transport inhibitor. AM404 inhibited anandamide transport with IC50 values of 1 and 5 μM in neurons and astrocytes, respectively. However, AM404 had no effect on FAAH activity or on uptake of arachidonate or ethanolamine. Also, AM404 exhibited affinity for CB1 receptors with Ki value of 1.8 μM [1]. In rat hepatic artery contracted with phenylephrine, AM404 evoked relaxations in a concentration-dependent way, which was inhibited by capsazepine, a vanilloid receptor antagonist. These results suggested that AM404 activated vanilloid receptors [2]. In SK-N-SH neuroblastoma cells, AM404 inhibited NFAT and NF-κB signaling pathways [3].
In mice, AM404 (10 mg/kg) significantly prolonged and enhanced anandamide-induced analgesia [1]. In rats, AM404 inhibited motor behaviors induced by quinpirole, a D2 family receptor agonist. In juvenile spontaneously hypertensive rats, AM404 inhibited hyperactivity [4].
References:
[1]. Beltramo M, Stella N, Calignano A, et al. Functional role of high-affinity anandamide transport, as revealed by selective inhibition. Science, 1997, 277(5329): 1094-1097.
[2]. Zygmunt PM, Chuang H, Movahed P, et al. The anandamide transport inhibitor AM404 activates vanilloid receptors. Eur J Pharmacol, 2000, 396(1): 39-42.
[3]. Caballero FJ, Soler-Torronteras R, Lara-Chica M, et al. AM404 inhibits NFAT and NF-κB signaling pathways and impairs migration and invasiveness of neuroblastoma cells. Eur J Pharmacol, 2015, 746: 221-232.
[4]. Beltramo M, de Fonseca FR, Navarro M, et al. Reversal of dopamine D(2) receptor responses by an anandamide transport inhibitor. J Neurosci, 2000, 20(9): 3401-3407.
| Cas No. | 198022-70-7 | SDF | |
| Synonyms | 4HPA, N(4hydroxyphenyl)Arachidonoyl Amide | ||
| Chemical Name | (5Z,8Z,11Z,14Z)-N-(4-hydroxyphenyl)icosa-5,8,11,14-tetraenamide | ||
| Canonical SMILES | O=C(CCC/C=C\C/C=C\C/C=C\C/C=C\CCCCC)NC(C=C1)=CC=C1O | ||
| Formula | C26H37NO2 | M.Wt | 395.58 |
| Solubility | <19.78mg/ml in ethanol; <19.78mg/ml in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5279 mL | 12.6397 mL | 25.2793 mL |
| 5 mM | 0.5056 mL | 2.5279 mL | 5.0559 mL |
| 10 mM | 0.2528 mL | 1.264 mL | 2.5279 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 38 reference(s) in Google Scholar.)
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