Ammonium tetrathiomolybdate(VI) |
Catalog No.GC67669
One-Click Copy Product Info
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Ammonium tetrathiomolybdate(VI) is an inorganic compound composed of ammonium cations and tetrathiomolybdate(VI) anions. Ammonium tetrathiomolybdate(VI) is commonly used in metabolic research of cardiovascular and cerebrovascular diseases and as a chelating agent for heavy metal detoxification.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 15060-55-6
Sample solution is provided at 25 µL, 10mM.
Ammonium tetrathiomolybdate(VI) is an inorganic compound composed of ammonium cations and tetrathiomolybdate(VI) anions. Ammonium tetrathiomolybdate(VI) is commonly used in metabolic research of cardiovascular and cerebrovascular diseases and as a chelating agent for heavy metal detoxification[1]. The chemical formula of Ammonium tetrathiomolybdate(VI) is (NH₄)₂MoS₄. Ammonium tetrathiomolybdate(VI) serves as a source of molybdenum in biological systems. Ammonium tetrathiomolybdate(VI) is particularly valuable in the study of molybdenum-dependent enzymes such as sulfite oxidase and xanthine oxidase[2]. The thiolate ligands in this compound enable Ammonium tetrathiomolybdate(VI) to act as a chelator, binding to heavy metals like copper and iron.The neuroprotective effects of Ammonium tetrathiomolybdate (VI) are receiving extensive attention in cerebral ischemia research[3]. Additionally, Ammonium tetrathiomolybdate(VI) copper chelation complexes exhibit antibacterial properties[4].
In vitro, AC16 cardiomyocytes were treated with Ammonium tetrathiomolybdate(VI) (20μM). Pre-treatment with Ammonium tetrathiomolybdate(VI) significantly increased cell viability, indicating Ammonium tetrathiomolybdate(VI) protective effect against copper-induced cytotoxicity[5]. In HeLa cells, treatment with Ammonium tetrathiomolybdate(VI) (20μM) failed to affect the increase in LC3-II levels induced by ML-SA5 (1μM) and could not reverse the autophagy-inhibiting effect of ML-SA5[6].
In vivo, a rat model of focal cerebral ischemia-reperfusion (tMCAO, ischemia for 90 minutes) was established. Ammonium tetrathiomolybdate(VI) (10mg/kg) was administered intravenously immediately before reperfusion, followed by continuous intravenous infusion at 10mg/kg/h for 60 minutes. This treatment improved the motor coordination and spontaneous movement duration of tMCAO mice and reduced the infarct area[7]. In a model of endometriosis induced by autologous uterine tissue transplantation in TNFR1⁻/⁻ female C57BL/6 mice, oral administration of Ammonium tetrathiomolybdate(VI) (0.3mg) for 1 month resulted in the chelation of copper ions, downregulation of estradiol levels and angiogenesis-related genes, inhibition of cell proliferation, and induction of oxidative stress. These effects collectively slowed the progression of endometriosis in TNFR1⁻/⁻ mice[8].
References:
[1] Zhang M, Qiu H, Mao L, et al. Ammonium tetrathiomolybdate triggers autophagy-dependent NRF2 activation in vascular endothelial cells. Cell Death Dis. 2022 Aug 25;13(8):733.
[2] Langlois DK, Querubin JR, Schall WD, et al. Ammonium tetrathiomolybdate treatment of copper-associated hepatopathy in dogs. J Vet Intern Med. 2019 May;33(3):1336-1343.
[3] Mendonça BP, Cardoso JDS, Michels M, et al. Neuroprotective effects of ammonium tetrathiomolybdate, a slow-release sulfide donor, in a rodent model of regional stroke. Intensive Care Med Exp. 2020 Apr 9;8(1):13.
[4] Zhang L, Tsai IC, Ni Z, et al. Copper Chelation Therapy Attenuates Periodontitis Inflammation through the Cuproptosis/Autophagy/Lysosome Axis. Int J Mol Sci. 2024 May 28;25(11):5890.
[5] Huo S, Wang Q, Shi W, et al. ATF3/SPI1/SLC31A1 Signaling Promotes Cuproptosis Induced by Advanced Glycosylation End Products in Diabetic Myocardial Injury. Int J Mol Sci. 2023 Jan 14;24(2):1667.
[6] Qi J, Xing Y, Liu Y, et al. MCOLN1/TRPML1 finely controls oncogenic autophagy in cancer by mediating zinc influx. Autophagy. 2021 Dec;17(12):4401-4422.
[7] Dyson A, Dal-Pizzol F, Ammonium tetrathiomolybdate following ischemia/reperfusion injury: Chemistry, pharmacology, and impact of a new class of sulfide donor in preclinical injury models. PLoS Med. 2017 Jul 5;14(7):e1002310.
[8] Conforti RA, Delsouc MB, Zabala AS, et al. The copper chelator ammonium tetrathiomolybdate inhibits the progression of experimental endometriosis in TNFR1-deficient mice. Sci Rep. 2023 Jun 26;13(1):10354.
| Cell experiment [1]: | |
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Cell lines |
AC16 cardiomyocytes |
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Preparation Method |
AC16 cardiomyocytes were seeded into 96-well plates with 5 × 103 cells per well and cultured for 24 hours. The cells were then treated with different concentrations (0, 10, 20, 50, and 100μM) of Ammonium tetrathiomolybdate(VI). Cell viability was assessed using the Cell Counting Kit-8 (CCK-8) assay after 48 hours of treatment. |
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Reaction Conditions |
0, 10, 20, 50, and 100μM; 48 hours |
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Applications |
Ammonium tetrathiomolybdate(VI) improved the cell viability of AC16 cardiomyocytes treated with copper ionophores and copper ions, Ammonium tetrathiomolybdate(VI) protective effect against copper-induced cytotoxicity |
| Animal experiment [2]: | |
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Animal models |
TNFR1⁻/⁻ female C57BL/6 mice |
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Preparation Method |
Female TNFR1⁻/⁻ C57BL/6 mice (2 months old) were used to induce endometriosis by autologous uterine tissue transplantation to the intestinal mesentery. From the 15th postoperative day, the mice received oral administration of 0.3mg Ammonium tetrathiomolybdate(VI) daily until sacrifice at 1 month post-induction. |
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Dosage form |
0.3mg/mouse daily for 2 weeks; oral gavage |
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Applications |
Ammonium tetrathiomolybdate(VI) reduced copper and estradiol levels in peritoneal fluid, decreased endometriotic lesion volume and weight, inhibited cell proliferation (PCNA-positive cells), reduced blood vessel density, and downregulated angiogenic genes (Vegfa, Fgf2, Pdgfb). |
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References: |
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| Cas No. | 15060-55-6 | SDF | |
| Formula | H8MoN2S4 | M.Wt | 260.27 |
| Solubility | DMSO : 5 mg/mL (19.21 mM; Need ultrasonic) | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.8422 mL | 19.2108 mL | 38.4216 mL |
| 5 mM | 768.4 μL | 3.8422 mL | 7.6843 mL |
| 10 mM | 384.2 μL | 1.9211 mL | 3.8422 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Assay: ＞99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)