Azaserine (Synonyms: CI-337,CN 15757,O-Diazoacetyl-L-serine,NSC 742) |
Catalog No.GC14874 |
tumor-inhibiting antibiotic
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 115-02-6
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
IC50: 7 μM: inhibits parasite growth.
Azaserine, as a naturally occurring serine derivative diazo compound, functions as a purine antagonist and structural analogue of glutamine that inhibits enzymatic activities involving in the pathways of glutamine metabolism. Azaserine, an antibiotic and antitumor agent, is used as a potential antineoplastic agent in clinical studies. Azaserine dampens the biosynthesis of purine via reacting with cysteine residues in the enzyme active sites. In addition, azaserine triggers DNA damage by the formation of carboxymethylated bases and O6-methylguanine.
In vitro: Azaserine showed cytotoxicity in Raji cells, which was partly due to inhibition of de novo purine biosynthesis, and the expression of O6-methylguanine-DNA methyltransferase did not provide protection against cell killing, suggesting that O6-methylguanine was not a major contributor to the cytotoxic DNA damage triggered by azaserine. Azaserine killed the Raji hypoxanthine-guanine phosphoribosyltransferas-deficient(HPRT-) Mex- cells. In contrast, the Raji HPRT+ Mex- cells were more resistant to azaserine. Additionally, azaserine blocked the growth of Raji HPRT+ Mex-cells when treated with 300 μM [1].
In vivo: CD-l mice and W/LEW rats were injected intraperitoneally with azaserine at a dose of 10 mg/kg body weight once a week for 5 weeks. After 6 months, compared to the control rats and mice, the azaserine-treated animals had a slightly higher incidence of pancreatic atypical acinar cell nodules (AACN) and the average size of AACN of azaserine-treated animals was larger. In addition, the concentration of [14C] azaserine and/or its metabolites was lower in mouse pancreas than in rat pancreas [2].
References:
[1]. O'Driscoll, M., Macpherson, P., Xu, Y., & Karran, P. The cytotoxicity of DNA carboxymethylation and methylation by the model carboxymethylating agent azaserine in human cells. Carcinogenesis. 1999; 20(9): 1855-1862.
[2]. B. D. Roebuck, Herman S. Lilja, Thomas J. Curphey, Daniel S. Longnecker; Pathologic and Biochemical Effects of Azaserine in Inbred Wistar/Lewis Rats and Noninbred CD-1 Mice. J Natl Cancer Inst. 1980; 65 (2): 383-389.
Cas No. | 115-02-6 | SDF | |
Synonyms | CI-337,CN 15757,O-Diazoacetyl-L-serine,NSC 742 | ||
Chemical Name | O-(2-diazoacetyl)-L-serine | ||
Canonical SMILES | O=C(C=[N+]=[N-])OC[C@H](N)C(O)=O | ||
Formula | C5H7N3O4 | M.Wt | 173.1 |
Solubility | ≥ 21.4 mg/mL in Water | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 5.777 mL | 28.885 mL | 57.7701 mL |
5 mM | 1.1554 mL | 5.777 mL | 11.554 mL |
10 mM | 0.5777 mL | 2.8885 mL | 5.777 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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