Azithromycin (Synonyms: CP 62,993) |
Catalog No.GC14507
One-Click Copy Product Info
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Antibiotic by inhibiting protein synthesis
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 83905-01-5
Sample solution is provided at 25 µL, 10mM.
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Azithromycin is a macrolide antibiotic useful for the treatment of a number of bacterial infections.
Azithromycin (2 μM) augments rhinovirus-induced IFNβ expression in primary bronchial epithelial cells from asthmatics, which is associated with over-expression of RIG-I like receptors and repression of viral replication. Knockdown of MDA5, but not knockdown of RIG-I, diminishes azithromycin (2 μM)-enhanced viral-induced IFNβ expression in asthmatic primary bronchial epithelial cells[1]. Azithromycin specifically reduces MMP-9 mRNA and protein levels without affecting NF-κB in endotoxin-challenged monocytic THP-1 cells[2].
Azithromycin (50 mg/kg) has no effect on bronchoalveolar lavage inflammatory parameters and LDH levels in a mouse model of asthma exacerbation. Azithromycin induces neither general inflammatory parameters nor LDH release in a mouse model of asthma exacerbation, and augments expression of interferon-stimulated genes and the pattern recognition receptor MDA5 but not RIG-I in exacerbating mice[1].
References:
[1]. Menzel M, et al. Azithromycin augments rhinovirus-induced IFNβ via cytosolic MDA5 in experimental models of asthma exacerbation. Oncotarget. 2017 Mar 18.
[2]. Vandooren J, et al. Differential inhibition of activity, activation and gene expression of MMP-9 in THP-1 cells by azithromycin and minocycline versus bortezomib: A comparative study. PLoS One. 2017 Apr 3;12(4):e0174853.
| Trypanocidal activity experiment [1]: | |
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Trypanosomes |
T. congolense, T. b. brucei and T. evansi |
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Preparation method |
The solubility of this compound in DMSO is > 30.1 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
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Reacting condition |
0.32 ~ 25 μM; 72 hrs |
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Applications |
The IC50 values of Azithromycin for T. congolense, T. b. brucei and T. evansi were 0.19 ± 0.17, 3.69 ± 2.26 and 1.81 ± 1.82 μM, respectively. |
| Animal experiment [1]: | |
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Animal models |
Mice infected with T. congolense |
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Dosage form |
50, 100, 200, 300 and 400 mg/kg; p.o. |
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Applications |
In mice infected with T. congolense, Azithromycin dose-dependently killed T. congolense, with the initial clearance of the parasites from the peripheral circulation in all treatment groups. However, this was followed by a relapse resulting in the rapid growth of the parasites. On the other hand, the survival rate was significantly prolonged in all treatment groups. A number of mice survived in the 200, 300 and 400 mg/kg Azithromycin groups, while all mice in the control and the 50 and 100 mg/kg Azithromycin groups died. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Molefe NI, Yamasaki S, Macalanda AMC, Suganuma K, Watanabe K, Xuan X, Inoue N. Oral administration of azithromycin ameliorates trypanosomosis in Trypanosoma congolense-infected mice. Parasitol Res. 2017 Jul 4. | |
| Cas No. | 83905-01-5 | SDF | |
| Synonyms | CP 62,993 | ||
| Canonical SMILES | CCC1C(C(C(N(CC(CC(C(C(C(C(C(=O)O1)C)OC2CC(C(C(O2)C)O)(C)OC)C)OC3C(C(CC(O3)C)N(C)C)O)(C)O)C)C)C)O)(C)O | ||
| Formula | C38H72N2O12 | M.Wt | 748.98 |
| Solubility | ≥ 30.05mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3351 mL | 6.6757 mL | 13.3515 mL |
| 5 mM | 267 μL | 1.3351 mL | 2.6703 mL |
| 10 mM | 133.5 μL | 667.6 μL | 1.3351 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 5 reference(s) in Google Scholar.)