BODIPY 493/503 methyl bromide |
Catalog No.GC68792
One-Click Copy Product Info
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BODIPY 493/503 methyl bromide is a fluorescent fatty acid analog with maximum excitation/emission wavelengths at 493/503nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 216434-81-0
Sample solution is provided at 25 µL, 10mM.
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BODIPY 493/503 methyl bromide is a fluorescent fatty acid analog with maximum excitation/emission wavelengths at 493/503nm[1]. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability. Its fluorescence peak is relatively sharp and its quantum yield is high. It is relatively insensitive to the polarity and pH value of the environment and is relatively stable under different physiological conditions[2]. Due to its asymmetric structure, BODIPY has a variety of derivative structural products[3]. BODIPY lipid droplet dyes can easily pass through the cell membrane and enter the cell interior. They can be specifically stained by localizing on neutral lipids in the cell, so the dye can be used to label living cells and fixed cells[4, 5].
References:
[1]Edmunds L R. Regulation of metabolism by the oncoprotein C-MYC[M]. University of Pittsburgh, 2015.
[2]Bumagina N A, Antina E V, Ksenofontov A A, et al. Basic structural modifications for improving the practical properties of BODIPY[J]. Coordination Chemistry Reviews, 2022, 469: 214684.
[3]Qin Y, Liu X, Jia P P, et al. BODIPY-based macrocycles[J]. Chemical Society Reviews, 2020, 49(16): 5678-5703.
[4]Jurgutis D, Jarockyte G, Poderys V, et al. Exploring BODIPY-Based sensor for Imaging of intracellular microviscosity in human breast cancer cells[J]. International Journal of Molecular Sciences, 2022, 23(10): 5687.
[5]Hsieh K, Lee Y K, Londos C, et al. Perilipin family members preferentially sequester to either triacylglycerol-specific or cholesteryl-ester-specific intracellular lipid storage droplets[J]. Journal of cell science, 2012, 125(17): 4067-4076.
This protocol is only a guide. Please modify it according to your specific needs.
1. Preparation of staining solution
(1) Stock solution: Dissolve BODIPY 493/503 methyl bromide in DMSO to prepare a stock solution with a concentration of 1-10mM.
Note: After unused stock solution is aliquoted, store it at -20°C or -80°C in the dark to avoid repeated freezing and thawing.
(2) Working solution: Dilute the stock solution with a suitable buffer (such as serum-free medium or PBS) to prepare a working solution with a concentration of 1-10μM.
Note: Please adjust the concentration of the working solution according to the actual situation and prepare it before use.
2. Cell staining (suspended cells)
(1) Collect cells by centrifugation and wash twice with PBS, each time for 5min.
(2) Add 1mL of BODIPY 493/503 methyl bromide working solution to resuspend the cells. The recommended cell density is 1×106/mL, incubate at room temperature in the dark for 5-30min. The optimal incubation time for different cells is different. Please explore it yourself according to the specific experimental needs.
(3) After the incubation is completed, centrifuge at 400g for 3-4min, remove the supernatant, and add PBS to wash 2-3 times, 5min each time.
(4) After resuspending the cells with 1mL of serum-free culture medium or PBS, use a fluorescence microscope or flow cytometer to observe.
3. Cell staining (adherent cells)
(1) Culture adherent cells on sterile coverslips.
(2) Remove the coverslip from the culture medium and aspirate the excess culture medium.
(3) Add 100μL of dye working solution from one corner of the coverslip and gently shake to evenly cover all cells with the dye.
(4) Incubate at room temperature in the dark for 5-30min. The optimal incubation time for different cells is different. Please explore it yourself according to the specific experimental needs.
(5) After the incubation is completed, aspirate the dye working solution, wash the coverslip with culture medium 2-3 times, and use a fluorescence microscope or flow cytometer to observe.
Notes:
(1) It is recommended to set up a positive control. The cells in the control group are incubated with 30μM oleic acid for 8h before subsequent experiments;
(2) All fluorescent dyes have quenching problems. Please try to avoid light to slow down fluorescence quenching;
(3) For your safety and health, please wear a lab coat and disposable gloves during operation.
| Cas No. | 216434-81-0 | SDF | |
| Formula | C14H16BBrF2N2 | M.Wt | 341 |
| Solubility | DMSO : 10 mg/mL (29.33 mM; ultrasonic and warming and heat to 60°C) | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9326 mL | 14.6628 mL | 29.3255 mL |
| 5 mM | 586.5 μL | 2.9326 mL | 5.8651 mL |
| 10 mM | 293.3 μL | 1.4663 mL | 2.9326 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)