Bz-FVR-AMC (trifluoroacetate salt) (Synonyms: Benzoyl-Phe-Val-Arg-AMC, Thrombin Fluorogenic Substrate III) |
| Catalog No.GC52488 |
A fluorogenic peptide substrate
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Bz-FVR-AMC is a fluorogenic peptide substrate for bovine cathepsins S and H, Xenopus endopeptidase XSCEP1, and esterase E-II from B. gabonica venom, among others.1,2,3 Upon enzymatic cleavage, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify enzyme activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.
1.Xin, X.-Q., Gunesekera, B., and Mason, R.W.The specificity and elastinolytic activities of bovine cathepsins S and HArch. Biochem. Biophys.299(2)334-339(1992) 2.Darby, N.J., Lackey, D.B., and Smyth, D.G.Purification of a cysteine endopeptidase which is secreted with bioactive peptides from the epidermal glands of Xenopus laevisEur. J. Biochem.195(1)65-70(1991) 3.Cloete, D., Viljoen, C.C., and Schabort, J.C.Deviation from Michaelis-Menten kinetics for esterase E-II from the venom of Bitis gabonica, using synthetic arginine amides as substratesToxicon21(6)857-869(1983)
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