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C527

Catalog No.GC13892

Inhibitor of USP1/USF1 complex

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C527 Chemical Structure

Cas No.: 192718-06-2

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5mg
$47.00
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25mg
$185.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Kinase experiment [1]:

In Vitro Deubiquitination Assays

Purified USP5 enzyme was purchased from Boston Biochem. UCH-L1 and UCH-L3 were as reported previously. USP12/46 was prepared in our laboratory as described. The in vitro enzymatic assays were performed as described previously using ubiquitin-AMC (Ub-7-amido-4methylcoumarin; Boston Biochem) as a substrate in a reaction buffer containing 20 mM HEPES-KOH (pH 7.8), 20 mM NaCl, 0.1 mg/ml ovalbumin, 0.5 mM EDTA and 10 mM dithiothreitol. The fluorescence was measured by FluoStar Galaxy Fluorometer (BMG Labtech). For the Ub-vinylsulfone (VS) assay, the proteins were incubated with Ub-VS (Boston Biochem) at 0.5 μM final concentration for 45 min at 30 °C, followed by the immunoblotting analysis.

Cell experiment [1]:

Cell lines

human U20S osteosarcoma cells, Hela cells

Preparation method

The solubility of this compound in DMSO is <2.93mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

0.5, 1 and 2 μM; 24 h

Applications

In human U20S osteosarcoma cells, C527 promoted ID1 degradation in a dose-dependent way. In Hela cells, C527 increased the levels of Ub-FANCD2 and Ub-FANCI. Pre-treatment with C527 also enhanced the cytoxicity of DNA damaging agents including Mitomyin C and Camptothecin. C527 inhibited Camptothecin induced the Rad51 foci formation and reduced homologous recombination (HR) activity.

References:

1.Mistry H, Hsieh G, Buhrlage SJ et al. Small-molecule inhibitors of USP1 target ID1 degradation in leukemic cells. Mol Cancer Ther. 2013 Dec;12(12):2651-62. doi: 10.1158/1535-7163.MCT-13-0103-T. Epub 2013 Oct 15.

Background

C527, also named heterocyclic tricyclic 1,4-dihydro-1,4-dioxo-1H-naphthalene, is an inhibitor of USP1 (deubiquitinating enzyme 1) / USF1 (USP1-associated factor 1) complex with IC50 value of 0.88 ± 0.03 μM in vivo. C527 is a pan-deubiquitinating enzyme inhibitor in vitro, with a high nanomolar IC50 for the USP1/UAF1 complex.
C527 inhibited the DUB activity of the USP12/USP46 complex and other DUB enzymes in vitro. However, the IC50 of C527 for these DUB enzymes was higher in comparison to USP1/UAF1 complex. C527 had considerably less inhibitory effect on UCH-L1 and UCH-L3, a different subclass of deubiquitinating enzymes, referred to as the ubiquitin C- terminal hydrolases, even though they are also cysteine proteases.
In several leukemic cell lines, C527 promote ID1 degradation, and cause cytotoxicity. In mouse osteosarcoma cells, C527 promotes the degradation of ID1 and the concurrent upregulation of p21. in human U20S osteosarcoma cells, C527 promoted the dose-dependent degradation of ID1. In Hela cells, C527 treatments caused an increase in the levels of Ub-FANCD2 and Ub-FANCI, and inhibited Camptothecin induced the Rad51 foci. Pre-treatment of hela cells with USP1 inhibitor caused an enhancement in the cytoxicity of Mitomycin C and Camptothecin .
Reference:
1.Mistry H, Hsieh G, Buhrlage SJ et al. Small-molecule inhibitors of USP1 target ID1 degradation in leukemic cells. Mol Cancer Ther. 2013 Dec;12(12):2651-62. doi: 10.1158/1535-7163.MCT-13-0103-T. Epub 2013 Oct 15.

Chemical Properties

Cas No. 192718-06-2 SDF
Chemical Name 2-(4-fluorophenyl)naphtho[2,3-d]oxazole-4,9-dione
Canonical SMILES O=C1C2=C(N=C(C3=CC=C(F)C=C3)O2)C(C4=CC=CC=C41)=O
Formula C17H8FNO3 M.Wt 293.25
Solubility <2.93mg/mL in DMSO Storage 4°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 3.4101 mL 17.0503 mL 34.1006 mL
5 mM 0.682 mL 3.4101 mL 6.8201 mL
10 mM 0.341 mL 1.705 mL 3.4101 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 35 reference(s) in Google Scholar.)

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