Chenodeoxycholic Acid-d4 (Synonyms: CDCA-d4)

Chenodeoxycholic acid-d₄ (CDCA-d₄) is intended for use as an internal standard for the quantification of CDCA by GC- or LC-MS. CDCA is a hydrophobic primary bile acid.¹ It is formed from cholesterol in the liver via a multistep process catalyzed by the cytochrome P450 (CYP) isoforms CYP7A1, CYP8B1, and CYP27A1. CDCA is a farnesoid X receptor (FXR) agonist that binds to FXRs in a TR-FRET assay (EC₅₀ = 13 µM) and induces FXR transactivation in a reporter assay.^2,3 It induces transcription of the gene encoding the Nrf2 target glutamate cysteine ligase (GCL) in primary hepatocytes and HepG2 cells when used at concentrations ranging from 25 to 100 µM.⁴

1.Fiorucci, S., and Distrutti, E.Chenodeoxycholic acid: An update on its therapeutic applicationsBile acids and their receptors. Handbook of experimental pharmacology256265-282(2019) 2.Ohinata, Y., Payer, B., O'Carroll, D., et al.Blimp1 is a critical determinant of the germ cell lineage in miceNature436207-213(2005) 3.Urizar, N.L., Liverman, A.B., Dodds, D.T., et al.A natural product that lowers cholesterol as an anatagonist ligand for FXRScience296(5573)1703-1706(2002) 4.Tan, K.P., Yang, M., and Ito, S.Activation of nuclear factor (erythroid-2 like) factor 2 by toxic bile acids provokes adaptive defense responses to enhance cell survival at the emergence of oxidative stressMol. Pharmacol.72(5)1380-1390(2007)