Collagenase, Type VII is a protein that catalyzes chemical processes and breaks the peptide bonds in collagen[1]. Collagenase increases the activity of inflammation-related pathways, such as cytokine-cytokine receptor interactions, IL-17 and TNF signaling pathways, reduces the expression of C3, Fga, and Slc4a1 proteins, and enhances the expression of Penk protein[2]. Collagenase has been widely used to induce cerebral hemorrhage in mice and to alter mechanical hyperalgesia[3].
Different tissue types call for different types of Collagenase: Collagenase I (Cat. No.: GC19589) is commonly used for softer tissues such as epithelium, lung, fat, and adrenal gland. Collagenase II (Cat. No.: GC19588) is preferred for dense or complex tissues such as liver, bone, heart, cartilage, and skeletal muscle. Collagenase III (Cat. No.: GC19590) is suitable for general dissociation of mammary gland and most mammalian tissues. Collagenase IV (Cat. No.: GC19591) is recommended for islet isolation or experiments requiring preservation of cell surface receptors, and Collagenase V (Cat. No.: GC19592) is often used for pancreatic islet isolation. When the tissue type is unclear or mixed digestion is performed, Collagenase IV can serve as a broad-spectrum first choice, but preliminary experiments are needed to verify its effects on the target cells and surface markers[4].
In vivo, injection of Collagenase (0.25U/µl) into the striatum at a flow rate of 0.4µl/min for 5 minutes induced severe neurological deficits in rats 24 hours later[5].
References:[1] Alipour H, Raz A, Zakeri S, et al. Therapeutic applications of collagenase (metalloproteases): A review[J]. Asian Pacific Journal of Tropical Biomedicine, 2016, 6(11): 975-981.
[2] Cao F, Guo Y, Zhang Q, et al. Integration of transcriptome resequencing and quantitative proteomics analyses of collagenase VII-induced intracerebral hemorrhage in mice[J]. Frontiers in Genetics, 2020, 11: 551065.
[3] Bai Q, Han Y, Khan S, et al. A Novel Endoplasmic Reticulum‐Targeted Metal–Organic Framework–Confined Ruthenium (Ru) Nanozyme Regulation of Oxidative Stress for Central Post‐Stroke Pain[J]. Advanced Healthcare Materials, 2024, 13(2): 2302526.
[4] Shekhter A B, Balakireva A V, Kuznetsova N V, et al. Collagenolytic enzymes and their applications in biomedicine[J]. Current medicinal chemistry, 2019, 26(3): 487-505.
[5] Xie J, Wang B, Wang L, et al. Intracerebral and intravenous transplantation represents a favorable approach for application of human umbilical cord mesenchymal stromal cells in intracerebral hemorrhage rats[J]. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 2016, 22: 3552.