Collagenase, Type VII (Synonyms: Type VII collagenase) |
Catalog No.GC26859
One-Click Copy Product Info
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Collagenase, Type VII is a protein that catalyzes chemical processes and breaks the peptide bonds in collagen.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9001-12-1
Sample solution is provided at 25 µL, 10mM.
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Collagenase, Type VII is a protein that catalyzes chemical processes and breaks the peptide bonds in collagen[1]. Collagenase increases the activity of inflammation-related pathways, such as cytokine-cytokine receptor interactions, IL-17 and TNF signaling pathways, reduces the expression of C3, Fga, and Slc4a1 proteins, and enhances the expression of Penk protein[2]. Collagenase has been widely used to induce cerebral hemorrhage in mice and to alter mechanical hyperalgesia[3].
Different tissue types call for different types of Collagenase: Collagenase I (Cat. No.: GC19589) is commonly used for softer tissues such as epithelium, lung, fat, and adrenal gland. Collagenase II (Cat. No.: GC19588) is preferred for dense or complex tissues such as liver, bone, heart, cartilage, and skeletal muscle. Collagenase III (Cat. No.: GC19590) is suitable for general dissociation of mammary gland and most mammalian tissues. Collagenase IV (Cat. No.: GC19591) is recommended for islet isolation or experiments requiring preservation of cell surface receptors, and Collagenase V (Cat. No.: GC19592) is often used for pancreatic islet isolation. When the tissue type is unclear or mixed digestion is performed, Collagenase IV can serve as a broad-spectrum first choice, but preliminary experiments are needed to verify its effects on the target cells and surface markers[4].
In vivo, injection of Collagenase (0.25U/µl) into the striatum at a flow rate of 0.4µl/min for 5 minutes induced severe neurological deficits in rats 24 hours later[5].
References:[1] Alipour H, Raz A, Zakeri S, et al. Therapeutic applications of collagenase (metalloproteases): A review[J]. Asian Pacific Journal of Tropical Biomedicine, 2016, 6(11): 975-981.
[2] Cao F, Guo Y, Zhang Q, et al. Integration of transcriptome resequencing and quantitative proteomics analyses of collagenase VII-induced intracerebral hemorrhage in mice[J]. Frontiers in Genetics, 2020, 11: 551065.
[3] Bai Q, Han Y, Khan S, et al. A Novel Endoplasmic Reticulum‐Targeted Metal–Organic Framework–Confined Ruthenium (Ru) Nanozyme Regulation of Oxidative Stress for Central Post‐Stroke Pain[J]. Advanced Healthcare Materials, 2024, 13(2): 2302526.
[4] Shekhter A B, Balakireva A V, Kuznetsova N V, et al. Collagenolytic enzymes and their applications in biomedicine[J]. Current medicinal chemistry, 2019, 26(3): 487-505.
[5] Xie J, Wang B, Wang L, et al. Intracerebral and intravenous transplantation represents a favorable approach for application of human umbilical cord mesenchymal stromal cells in intracerebral hemorrhage rats[J]. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 2016, 22: 3552.
Collagenase is used for tissue extraction
1. Preparation of storage solution
(1) Add 1ml of calcium-magnesium-containing Hank's Balanced Salt Solution (HBSS) directly to the vial containing Collagenase (1g). Gently vortex to ensure complete dissolution and prepare a 1g/ml stock solution.
(2) Use a low-protein adsorption filtration device to filter and sterilize the stock solution through a 0.22μm filter membrane. Use immediately or aliquot and store in the dark at -20°C.
(3) Thaw before use. The commonly used concentration for tissue and cell dispersion is 0.5-2.5mg/ml, and the commonly used concentration for cartilage digestion is 1-2mg/ml. However, the optimal working concentration needs to be determined based on specific experimental conditions or by referring to relevant literature.
2. Tissue separation
(1) Use a sterile scalpel or scissors to cut the tissue into 3-4mm small pieces.
(2) Rinse the tissue fragments with calcium-magnesium-containing HBSS solution repeatedly.
(3) Add an adequate amount of calcium-magnesium-containing HBSS solution to fully immerse the tissue. Add the Collagenase solution to the desired working concentration.
(4) Incubate at 37°C for 4-18 hours. Use a shaker and add CaCl2 (at a final concentration of 3mM) to the digestion solution to improve efficiency.
(5) Disperse the cells through a sterile stainless steel or nylon mesh filter. The remaining tissue fragments can be added to fresh collagenase solution and incubated at 37°C to further dissociate.
(6) Centrifuge the dispersed cells several times with collagen-free HBSS solution.
(7) After the final washing step, resuspend the cell precipitate in the culture medium. Use an automatic cell counter to determine the live cell density (or use other automatic or manual methods).
(8) Suspend the cells in a suitable culture medium and inoculate into a culture vessel.
3. Important Notes
(1) To ensure your safety and health, please wear the laboratory coat and disposable gloves during the operation.
(2) This experimental protocol and all reagents are for scientific research purposes only.
References:[1] Xie J, Wang B, Wang L, et al. Intracerebral and intravenous transplantation represents a favorable approach for application of human umbilical cord mesenchymal stromal cells in intracerebral hemorrhage rats[J]. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 2016, 22: 3552.
| Cas No. | 9001-12-1 | SDF | |
| Synonyms | Type VII collagenase | ||
| Formula | M.Wt | ||
| Solubility | H2O : ≥ 50 mg/mL | Storage | Store at 2-8°C,protect from light |
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Biological Activity: ≥1000u/mg solid Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)