Corticosterone-d8 (Synonyms: 17-deoxy Cortisol-d8, 11β,21-DHP-d8, 11β,21-dihydroxy Progesterone-d8) |
Catalog No.GC45724
One-Click Copy Product Info
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Corticosterone-d8, the deuterated form of natural corticosterone, possesses glucocorticoid and mineralocorticoid activity.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1271728-07-4
Sample solution is provided at 25 µL, 10mM.
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Corticosterone-d8, the deuterated form of natural corticosterone, possesses glucocorticoid and mineralocorticoid activity. As a labeled tracer, Corticosterone-d8 is widely used to study the mechanisms of stress responses and assess stress levels. Corticosterone-d8 can also be quantitatively analyzed in biological samples via GC-MS or LC-MS to investigate its role in metabolic regulation[1].
References:
[1] Wang Y, Sun C, Cao Y, et al. Glycyrrhizic acid and patchouli alcohol in Huoxiang Zhengqi attenuate intestinal inflammation and barrier injury via regulating endogenous corticosterone metabolism mediated by 11β-HSD1. J Ethnopharmacol. 2025 Feb 10;338(Pt 1):119025.
Determination of 11β-hydroxysteroid dehydrogenase (11β-HSD) Oxidase Activity Using Corticosterone-d8[1]
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
1.Reagent Preparation: Dissolve Corticosterone-d8 in a suitable organic solvent (e.g., methanol or DMSO) to prepare a stock solution of the desired concentration. Dilute it to the working concentration (e.g., 4μM) before use. Prepare a buffer solution containing 20mM NADP⁺ (for measuring 11β-HSD1) or 20mM NAD⁺ (for measuring 11β-HSD2). Prepare colon tissue homogenate or human recombinant 11β-HSD1 (hr11β-HSD1) protein.
2.Sample Preparation: Take colon tissue, add pre-cooled homogenization buffer, and homogenize thoroughly. Centrifuge and collect the supernatant as the enzyme source. Alternatively, use the recombinant hr11β-HSD1 protein solution directly.
3.Establishment of the Enzymatic Reaction System: Add the following sequentially to the reaction system: tissue homogenate or hr11β-HSD1 protein solution, 20μM NADP⁺ (or NAD⁺) cofactor, and 4μM Corticosterone-d8 working solution.
4.Detection of 11β-HSD Oxidase Activity: Incubate the reaction system at 37°C for 1 hour, allowing Corticosterone-d8 to be oxidized by 11β-HSD enzyme to its product, 11-dehydrocorticosterone-d8 (11-DHC-d8). After the reaction, add a stop solution (e.g., organic solvent) to terminate the reaction. Centrifuge the reaction mixture and collect the supernatant. Use a liquid chromatography-mass spectrometry (LC-MS) system in multiple reaction monitoring (MRM) mode to specifically detect the peak area of the generated 11-DHC-d8. Enzyme activity is calculated and expressed as the amount of 11-DHC-d8 formed per hour per milligram of protein (formed/h/mg protein).
Precautions:
(1) This method utilizes a stable isotope-labeled substrate (Corticosterone-d8), which can effectively distinguish interference from endogenous corticosterone, improving the specificity and accuracy of detection. It is suitable for measuring enzyme activity in complex biological samples.
(2) Reaction time, substrate concentration, cofactor concentration, and pH value need to be optimized to ensure the reaction proceeds within the linear range.
(3) Corticosterone-d8 is a deuterated compound and should be stored properly, avoiding repeated freeze-thaw cycles. The experiment involves biological samples and chemical reagents. Operations should be performed in a compliant laboratory environment while wearing a lab coat, gloves, and necessary protective equipment.
References:
[1] Wang Y, Sun C, Cao Y, et al. Glycyrrhizic acid and patchouli alcohol in Huoxiang Zhengqi attenuate intestinal inflammation and barrier injury via regulating endogenous corticosterone metabolism mediated by 11β-HSD1. J Ethnopharmacol. 2025 Feb 10;338(Pt 1):119025.
| Cas No. | 1271728-07-4 | SDF | |
| Synonyms | 17-deoxy Cortisol-d8, 11β,21-DHP-d8, 11β,21-dihydroxy Progesterone-d8 | ||
| Canonical SMILES | O=C1C([2H])([2H])C[C@@]2(C)C(C([2H])([2H])C[C@]3([H])[C@]2([H])[C@@H](O)C[C@@]4(C)[C@@]3([H])CC[C@]4([2H])C(C([2H])([2H])O)=O)=C1[2H] | ||
| Formula | C21H22D8O4 | M.Wt | 354.5 |
| Solubility | DMSO : 100 mg/mL (282.08 mM; Need ultrasonic); DMSO : 100 mg/mL (282.08 mM; Need ultrasonic and warming); Ethanol : 14.29 mg/mL (40.31 mM; Need ultrasonic); H2O : 0.67 mg/mL (1.89 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8209 mL | 14.1044 mL | 28.2087 mL |
| 5 mM | 564.2 μL | 2.8209 mL | 5.6417 mL |
| 10 mM | 282.1 μL | 1.4104 mL | 2.8209 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 10 reference(s) in Google Scholar.)