Cy3-dUTP (Synonyms: Aminoallyl-dUTP - Cy3; 5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with Cy3, Triethylammonium salt; Cy3 dUTP) |
Catalog No.GC16524
One-Click Copy Product Info
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Cy3-dUTP is a Cyanine3-labeled dUTP fluorescent dye. Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554nm, λem=568nm). Cy3-dUTP can serve as a substrate for T4 and Taq DNA polymerases, Escherichia coli DNA polymerases (whole enzymes and Klenow fragments), reverse transcriptases (from AMV and M-MuLV), and terminal transferases.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Cy3-dUTP is a Cyanine3-labeled dUTP fluorescent dye. Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554nm, λem=568nm). Cy3-dUTP can serve as a substrate for T4 and Taq DNA polymerases, Escherichia coli DNA polymerases (whole enzymes and Klenow fragments), reverse transcriptases (from AMV and M-MuLV), and terminal transferases[1][2][3].
References:
[1] Yu H, Chao J, Patek D, et al. Cyanine dye dUTP analogs for enzymatic labeling of DNA probes. Nucleic Acids Res. 1994 Aug 11;22(15):3226-32.
[2] Jiang Y, Li Z S, Jiang F S,et al. Effects of different ingredients of zedoary on gene expression of HSC-T6 cells. World J Gastroenterol. 2005 Nov 21;11(43):6780-6.
[3] Gutjahr A, Xu S Y. Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA. Nucleic Acids Res. 2014 May;42(9):e77.
| Cas No. | SDF | ||
| Synonyms | Aminoallyl-dUTP - Cy3; 5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with Cy3, Triethylammonium salt; Cy3 dUTP | ||
| Chemical Name | 1-ethyl-2-((1E,3E)-3-(1-(6-(((E)-3-(1-((2R,4S,5R)-4-hydroxy-5-(((hydroxy((hydroxy(phosphonooxy)phosphoryl)oxy)phosphoryl)oxy)methyl)tetrahydrofuran-2-yl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)allyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-yli | ||
| Canonical SMILES | O=S(C1=CC=C([N+](CC)=C(C2(C)C)/C=C/C=C(C(C)(C)C3=C4C=CC(S(O)(=O)=O)=C3)/N4CCCCCC(NC/C=C/C5=CN([C@H]6C[C@H](O)[C@@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O6)C(NC5=O)=O)=O)C2=C1)([O-])=O | ||
| Formula | C43H56N5O21P3S2(free acid) | M.Wt | 1135.97 g/mol (free acid) |
| Solubility | Soluble in DMSO | Storage | Store at -80°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 880.3 μL | 4.4015 mL | 8.803 mL |
| 5 mM | 176.1 μL | 880.3 μL | 1.7606 mL |
| 10 mM | 88 μL | 440.2 μL | 880.3 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
Average Rating: 5 (Based on Reviews and 17 reference(s) in Google Scholar.)