Dantrolene-d4 |
Catalog No.GC46125
One-Click Copy Product Info
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A neuropeptide with diverse biological activities
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2749234-17-9
Sample solution is provided at 25 µL, 10mM.
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Dantrolene-d4 is intended for use as an internal standard for the quantification of dantrolene by GC- or LC-MS. Dantrolene is an inhibitor of calcium release from the sarcoplasmic reticulum (IC50 = 0.3 μM).1 It binds to sarcoplasmic reticulum vesicles isolated from normal and malignant hyperthermia-susceptible (MHS) pigs with Kd values of 0.3 and 0.09 μM, respectively. Dantrolene reduces spontaneous calcium wave frequency and amplitude in the presence of calmodulin in isolated mouse cardiomyocytes with IC50 values of 0.42 and 0.19 μM, respectively.2 It reduces the magnitude of electrically stimulated twitch tensions in isolated rat extensor digitorum longus and soleus muscles (IC50 = 3 μg/ml) and MHS pigs (ED50 = 0.85 mg/kg).3,4. Formulations containing dantrolene have been used in the treatment of malignant hyperthermia.
|1. Kobayashi, S., Yano, M., Suetomi, T., et al. Dantrolene, a therapeutic agent for malignant hyperthermia, markedly improves the function of failing cardiomyocytes by stabilizing interdomain interactions within the ryanodine receptor. J. Am. Coll. Cardiol. 53(21), 1993-2005 (2009).|2. Oo, Y.W., Gomez-Hurtado, N., Walweel, K., et al. Essential role of calmodulin in RyR inhibition by dantrolene. Mol. Pharmacol. 88(1), 57-63 (2015).|3. Kotsias, B.A., and Muchnik, S. Reversible effect of dantrolene sodium on twitch tension of rat skeletal muscle. Arch. Neurol. 35(4), 234-236 (1978).|4. Nelson, T.E., and Flewellen, E.H. Rationale for dantrolene vs. procainamide for treatment of malignant hyperthermia. Anesthesiology 50(2), 118-122 (1979).
| Cas No. | 2749234-17-9 | SDF | |
| Canonical SMILES | O=C(NC(C1)=O)N1/N=C/C2=CC=C(C3=C([2H])C([2H])=C([N+]([O-])=O)C([2H])=C3[2H])O2 | ||
| Formula | C14H6D4N4O5 | M.Wt | 318.3 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1417 mL | 15.7085 mL | 31.4169 mL |
| 5 mM | 628.3 μL | 3.1417 mL | 6.2834 mL |
| 10 mM | 314.2 μL | 1.5708 mL | 3.1417 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 27 reference(s) in Google Scholar.)