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Emodin (Synonyms: Archin, Frangulic Acid, NSC 408120, NSC 622947, Schuttgelb)

Katalog-Nr.GC16044

Natural CK2 inhibitor and ER agonist

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Emodin Chemische Struktur

Cas No.: 518-82-1

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10mM (in 1mL DMSO)
27,00 $
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100mg
35,00 $
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200mg
60,00 $
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

Epithelial ovarian cancer (EOC) cells

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

0 ~ 80 μM; 48 hrs

Applications

In a series of EOC cells, Emodin inhibited cell proliferation in a dose-dependent manner. Emodin at the dose of 20 μM abrogated the migration and invasion abilities of A2780 and SK-OV-3 cells transfected with pLVX-ILK. The results of western blotting analysis implied that Emodin inhibited the migration and invasion abilities of EOC cells by blocking epithelial-mesenchymal transition (EMT) through targeting integrin-linked kinase (ILK).

Animal experiment [1]:

Animal models

Nude mice bearing SK-OV-3/pLVX-ILK cells

Dosage form

50 mg/kg/d; i.p.; for 21 days

Applications

In nude mice bearing SK-OV-3/pLVX-ILK cells, Emodin significantly inhibited tumor growth. Meanwhile, Emodin counteracted the effects of over-expression of ILK on EOC cells. The immunohistochemical results demonstrated that Emodin inhibited the EMT of tumor cells. In addition, Emodin exhibited mild cardiac, liver and renal toxicities in vivo.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Lu J, Xu Y, Zhao Z, Ke X, Wei X, Kang J, Zong X, Mao H, Liu P. Emodin suppresses proliferation, migration and invasion in ovarian cancer cells by down regulating ILK in vitro and in vivo. Onco Targets Ther. 2017 Jul 19;10:3579-3589.

Background

Description: IC50 Value: observed at 72 hr were as follows: 66.9 uM, Hep3B cells; 74.36 uM, HepG2 cells; and 101.5 uM, Huh7 cells [1]. Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a naturally occurring anthraquinone present in the roots and barks of numerous plants, molds, and lichens, and an active ingredient of various Chinese herbs. Emodin exerts antiproliferative effects in cancer cells that are regulated by different signaling pathways. in vitro: At 6h after emodin treatment, the levels of GDF15, CYP1A1, CYP1B1, and CYR61 were upregulated [1]. Emodin increased the resting tension of gallbladder smooth muscle strips and inhibited voltage-dependent K(+) current in a dose-dependent manner. When 10 microM emodin was applied to gallbladder smooth muscle cells for 3-6 min., the amplitude of voltage-dependent K(+) current was decreased by 31.5 +/- 0.5% at +40 mV, and this inhibitory effect mostly recovered after washout [2]. in vivo: Emodin treatment significantly alleviated the severity of the disease, based on the reduced hind paw swelling and clinical scores, compared with untreated CIA mice. Comparing with untreated CIA mice, emodin treatment inhibited the levels of TNF-α and IL-6 in the plasma, PGE2 production, and COX-2 protein expression in synovial tissues in a dose manner [3]. Clinical trial: N/A

Chemical Properties

Cas No. 518-82-1 SDF
Überlieferungen Archin, Frangulic Acid, NSC 408120, NSC 622947, Schuttgelb
Chemical Name 1,3,8-trihydroxy-6-methylanthracene-9,10-dione
Canonical SMILES CC1=CC(O)=C2C(C(C3=CC(O)=CC(O)=C3C2=O)=O)=C1
Formula C15H10O5 M.Wt 270.24
Löslichkeit ≥ 27 mg/mL in DMSO Storage Store at 2-8°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 3.7004 mL 18.5021 mL 37.0041 mL
5 mM 0.7401 mL 3.7004 mL 7.4008 mL
10 mM 0.37 mL 1.8502 mL 3.7004 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

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