DRAQ5 |
| Catalog No.GC71783 |
DRAQ5 is a novel cell permeant and far red-fluorescing DNA probe.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 254098-36-7
Sample solution is provided at 25 µL, 10mM.
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Mammalian cell in full culture medium staining methods[2]:
(1) Cell planking: Digestive separation of cells and resuspend in complete medium to a concentration of 2-4 × 105 cells/ml.
Note: Attached cell cultures (e.g., coverslip cultures or chambered wells) can be stained in a 1-2-ml staining volume overlayering a 4-cm2 surface area.
(2) Prepare staining solution: Add 4 µl of 5 mM DRAQ5 acidified stock per ml culture medium (20 µM final).
Note: Nuclear discrimination is achievable at 2.5 to 5 µM, and it is unlikely that concentrations >30 µM would be required.
(3) Fluorescence staining: Incubate 5 to 15 min at 37°C.
Note: Overstaining cannot occur.
(4)Wash (optional): Centrifuge cells 3 to 5 min at 800 × g, 37°C. Discard supernatant and resuspend in complete medium with 10 mM HEPES at 4 × 105 cells/ml.
(5) For flow cytometry: Use conventional pulse analysis for doublet discrimination and analyze parameters using appropriate software.
(6) For laser scanning microscopy: Collect fluorescence images using a 695 nm long-pass filter.
Fixed cells staining methods[2]:
(1) Fixed cells:Use 4% paraformaldehyde in PBS for 30 min with resuspension in an aqueous buffer (e.g., PBS).
(2) Fluorescence staining: similar concentrations of dye and similar incubation conditions can be used as for live cells.
References:
[1]. Smith PJ, et al. A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometry. J Immunol Methods. 1999 Oct 29;229(1-2):131-9.
[2]. Smith PJ, et al. DRAQ5 labeling of nuclear DNA in live and fixed cells. Curr Protoc Cytom. 2004 May;Chapter 7:Unit 7.25.
| Cas No. | 254098-36-7 | SDF | |
| Formula | M.Wt | 412.5 | |
| Solubility | Storage | 4°C, protect from light | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.4242 mL | 12.1212 mL | 24.2424 mL |
| 5 mM | 0.4848 mL | 2.4242 mL | 4.8485 mL |
| 10 mM | 0.2424 mL | 1.2121 mL | 2.4242 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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