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EthD-III

Catalog No.GC71779

EthD-III is a nucleic acid probe.

Products are for research use only. Not for human use. We do not sell to patients.

EthD-III Chemical Structure

Size Price Stock Qty
1 mg
$1,822.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Description of EthD-III

EthD-III is a nucleic acid probe. EthD-III is a red fluorescent stain that can be used to detect dead cells. EthD-III enters cells with damaged membranes and binds to nucleic acids, resulting in bright red fluorescence in dead cells (Ex/Em=530/645 nm).

References:
[1]. Anastassov I, et al. Cytoprotection by endogenous zinc in the vertebrate retina. J Neurochem. 2014 Apr;129(2):249-55.
[2]. Horie T, et al. OmpA-Like Proteins of Porphyromonas gingivalis Mediate Resistance to the Antimicrobial Peptide LL-37. J Pathog. 2018 Dec 27;2018:2068435.

Chemical Properties of EthD-III

Cas No. SDF
Formula C51H62Cl5N9 M.Wt 978.36
Solubility Storage
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of EthD-III

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 1.0221 mL 5.1106 mL 10.2212 mL
5 mM 0.2044 mL 1.0221 mL 2.0442 mL
10 mM 0.1022 mL 0.5111 mL 1.0221 mL
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**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

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In vivo Formulation Calculator (Clear solution) of EthD-III

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Product Documents

Quality Control & SDS

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Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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