Autoinducing Peptide I |
Catalog No.GA20788
One-Click Copy Product Info
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Autoinducing Peptide I is a quorum-sensing autoinducing peptide secreted by Staphylococcus aureus.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 200010-29-3
Sample solution is provided at 25 µL, 10mM.
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Autoinducing Peptide I is a quorum-sensing autoinducing peptide secreted by Staphylococcus aureus[1]. Autoinducing Peptide I acts as a specific agonist of the AgrC histidine kinase receptor and potently activates the agr system[2]. Upon binding and activating AgrC, Autoinducing Peptide I triggers the expression of virulence genes including RNAIII, and regulates the production of bacterial exotoxins, hemolysins, and other pathogenic factors[3]. Autoinducing Peptide I is widely used in the study of bacterial quorum sensing and virulence regulation mechanisms[4][5]. Autoinducing Peptide I is also used in studies of anti-infective mechanisms and novel antibacterial adjuvants, providing structural templates and target validation for the development of new antimicrobial therapies[6][7].
References:
[1] Lyon GJ, Wright JS, Muir TW, Novick RP. Key determinants of receptor activation in the agr autoinducing peptides of Staphylococcus aureus. Biochemistry. 2002;41(31):10095-10104.
[2] Wang B, Zhao A, Novick RP, Muir TW. Activation and inhibition of the receptor histidine kinase AgrC occurs through opposite helical transduction motions. Mol Cell. 2014;53(6):929-940.
[3] Gordon CP, Olson SD, Lister JL, Kavanaugh JS, Horswill AR. Truncated Autoinducing Peptides as Antagonists of Staphylococcus lugdunensis Quorum Sensing. J Med Chem. 2016;59(19):8879-8888.
[4] Arvidson S, Tegmark K. Regulation of virulence determinants in Staphylococcus aureus. Int J Med Microbiol. 2001;291(2):159-170.
[5] Tal-Gan Y, Stacy DM, Foegen MK, Koenig DW, Blackwell HE. Highly potent inhibitors of quorum sensing in Staphylococcus aureus revealed through a systematic synthetic study of the group-III autoinducing peptide. J Am Chem Soc. 2013;135(21):7869-7882.
[6] Daly SM, Joyner JA, Triplett KD, et al. VLP-based vaccine induces immune control of Staphylococcus aureus virulence regulation. Sci Rep. 2017;7(1):637.
[7] Tal-Gan Y, Ivancic M, Cornilescu G, Cornilescu CC, Blackwell HE. Structural characterization of native autoinducing peptides and abiotic analogues reveals key features essential for activation and inhibition of an AgrC quorum sensing receptor in Staphylococcus aureus. J Am Chem Soc. 2013;135(49):18436-18444.
| Cas No. | 200010-29-3 | SDF | |
| Formula | C43H60N8O13S2 | M.Wt | 961.11 |
| Solubility | DMSO : 100 mg/mL (104.05 mM; Need ultrasonic) | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.0405 mL | 5.2023 mL | 10.4046 mL |
| 5 mM | 208.1 μL | 1.0405 mL | 2.0809 mL |
| 10 mM | 104 μL | 520.2 μL | 1.0405 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 35 reference(s) in Google Scholar.)