Big Endothelin-3 (1-41) amide (human) |
Catalog No.GA20810
One-Click Copy Product Info
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Big Endothelin-3 (1-41) amide (human) is a precursor molecule of the Endothelin (ET)-3 peptide composing of 41 amino acids.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 133551-97-0
Sample solution is provided at 25 µL, 10mM.
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Big Endothelin-3 (1-41) amide (human) is a precursor molecule of the Endothelin (ET)-3 peptide composing of 41 amino acids [1]. Big Endothelin-3 (1-41) amide can be converted into the mature form by ET converting enzyme, increasing the level of immunoreactive endothelin (IR-ET) in vascular smooth muscle cells (VSMC)[2]. Big Endothelin-3 (1-41) amide has been widely used to regulate the nociceptive behavioral responses of mice[3].
In vitro, Big Endothelin-3 (1-41) amide treatment via intravenous injection at 3nmol/kg within 60min produced a long-lasting hypertensive action in ganglion-blocked anaesthetized rats [4].
References:
[1] Davenport A P, Hyndman K A, Dhaun N, et al. Endothelin[J]. Pharmacological reviews, 2016, 68(2): 357-418.
[2] Tsukahara Y, Matsumura Y, Kuninobu K, et al. Phosphoramidon-sensitive endothelin converting enzyme in cultured vascular smooth muscle cells converts big endothelin-3 to endothelin-3[J]. Life sciences, 1993, 53(6): 465-471.
[3] Raffa R B, Schupsky J J, Jacoby H I. Endothelin-induced nociception in mice: mediation by ETA and ETB receptors[J]. The journal of pharmacology and experimental therapeutics, 1996, 276(2): 647-651.
[4] Matsumura Y, Fujita K, Takaoka M, et al. Big endothelin-3-induced hypertension and its inhibition by phosphoramidon in anaesthetized rats[J]. European journal of pharmacology, 1993, 230(1): 89-93.
| Animal experiment [1]: | |
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Animal models |
Male Sprague-Dawley rats |
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Preparation Method |
Male Sprague-Dawley rats weighing 280-330g were anaesthetized with sodium thiobutabarbital (100mg/kg; i.p.) and placed on a heated surgical tray that maintained the rectal temperature between 37-38°C. After tracheotomy, the right femoral vein was cannulated for bolus injection of drugs. The right femoral artery was cannulated for blood pressure (BP) measurement with a pressure transducer. Rats were ganglion-blocked with pentolinium (5mg/kg; i.v.). After a 30-rain equilibration period, ET-3 or Big Endothelin-3 (1-41) amide, dissolved in 0.9% saline containing 0.1% heat-inactivated bovine serum albumin, was injected i.v. (3nmol/kg; 0.3ml). Record the blood pressure changes in rats within 60 minutes. |
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Dosage form |
3nmol/kg for once; i.v. |
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Applications |
Big Endothelin-3 (1-41) amide treatment produced a long-lasting hypertensive action in rats. |
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References: |
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| Cas No. | 133551-97-0 | SDF | |
| Formula | C223H322N56O63S4 | M.Wt | 4923.61 |
| Solubility | 0.2-0.5mg/ml in 0.1% Acetic acid | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 203.1 μL | 1.0155 mL | 2.031 mL |
| 5 mM | 40.6 μL | 203.1 μL | 406.2 μL |
| 10 mM | 20.3 μL | 101.6 μL | 203.1 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 33 reference(s) in Google Scholar.)