Cyclolinopeptide B |
Catalog No.GA21349
One-Click Copy Product Info
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Cyclolinopeptide B is a cyclic nonapeptide with significant biological activity.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 193139-41-2
Sample solution is provided at 25 µL, 10mM.
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Cyclolinopeptide B is a cyclic nonapeptide with significant biological activity [1]. Cyclolinopeptide B inhibits the activation of T cells and B cells and downregulates the expression of immune-related genes (such as IL-2 and IFN-γ) [2]. Cyclolinopeptide B is commonly used to treat cancer [3].
In Sk-Br-3 cells, Cyclolinopeptide B (25, 50, 100, 200, 400μg/mL; 24h, 48h) has concentration dependent cytotoxicity [4]. In human gastric SGC7901 cells, Cyclolinopeptide B (40, 80, 120, 160, 200, 240μM; 24h, 48h) induces apoptosis by activating Fas, FasL, caspase 8, and caspase 3 [5]. In human gastric SGC7901 cells, Cyclolinopeptide B (80, 120, 200μM; 24h) isolated from flaxseed induce G1 cell cycle arrest on SGC-7901 cells by modulating the AKT/JNK signaling pathway [6].
References:
[1]. Morita H, Shishido A, Matsumoto T, et al. A new immunosuppressive cyclic nonapeptide, cyclolinopeptide B from Linum usitatissimum. Bioorganic & Medicinal Chemistry Letters. 1997 May 20; 7(10): 1269-1272.
[2]. Aladedunye F, Sosinska E, Przybylski R. Flaxseed cyclolinopeptides: analysis and storage stability. Journal of the American Oil Chemists' Society. 2013 Mar; 90: 419-428.
[3]. Fojnica A, Gromilic Z, Vranic S, et al. Anticancer potential of the cyclolinopeptides. Cancers. 2023 Jul 30; 15(15): 3874.
[4]. Okinyo-Owiti DP, Dong Q, Ling B, et al. Evaluating the cytotoxicity of flaxseed orbitides for potential cancer treatment. Toxicology reports. 2015 Jan 1; 2: 1014-1018.
[5]. Zou XG, Hu JN, Li J, et al. iCellular uptake of [1–9-NαC]-linusorb B2 and [1–9-NαC]-linusorb B3 isolated from flaxseed, and their antitumor activities in human gastric SGC-7901 cells. Journal of Functional Foods. 2018 Sep 1; 48: 692-703.
[6]. Zou XG, Hu JN, Wang M, et al. [1–9-NαC]-linusorb B2 and [1–9-NαC]-linusorb B3 isolated from flaxseed induce G1 cell cycle arrest on SGC-7901 cells by modulating the AKT/JNK signaling pathway. Journal of Functional Foods. 2019 Jan 1; 52: 332-339.
| Cell experiment [1]: | |
Cell lines | Sk-Br-3 cells |
Preparation Method | The cultured cells were inoculated in 96-well plates, with 10,000 cells per well for the Sk-Br-3 cell line. After the inoculated cells grew to 70-80% confluence, they were treated with LOB for 24 hours and 48 hours, respectively. For Sk-Br-3 cells, the Cyclolinopeptide B administration concentrations were 400μg/mL, 200, 100, 50, and 25μg/mL, respectively. |
Reaction Conditions | 25, 50, 100, 200, 400μg/mL; 24h, 48h |
Applications | Cyclolinopeptide B has concentration dependent cytotoxicity in Sk-Br-3 cells. |
[1]. Okinyo-Owiti DP, Dong Q, Ling B, et al. Evaluating the cytotoxicity of flaxseed orbitides for potential cancer treatment. Toxicology reports. 2015 Jan 1; 2: 1014-1018. | |
| Cas No. | 193139-41-2 | SDF | |
| Formula | C56H83N9O9S | M.Wt | 1058.4 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 944.8 μL | 4.7241 mL | 9.4482 mL |
| 5 mM | 189 μL | 944.8 μL | 1.8896 mL |
| 10 mM | 94.5 μL | 472.4 μL | 944.8 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)