H-Gly-Arg-AMC |
Catalog No.GA22410
One-Click Copy Product Info
|
H-Gly-Arg-AMC is a fluorogenic substrate for cathepsin C, with maximum excitation/emission wavelengths of 340-360/440-460nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 65147-19-5
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
H-Gly-Arg-AMC is a fluorogenic substrate for cathepsin C, with maximum excitation/emission wavelengths of 340-360/440-460nm. When cleaved by cathepsin C, 7-amino-4-methylcoumarin (AMC) is liberated, and its fluorescence serves to measure the activity of cathepsin C[1]. H-Gly-Arg-AMC contains the AMC group and can be used to assess the activity of various proteases and peptidases. AMC has been widely used for monitoring the activity of aminopeptidases, dipeptidases, and carboxypeptidases. The H- can improve the water solubility of peptide substrates, optimize the kinetic parameters of the substrates (such as KM and kcat), enhance the specificity of the substrates, and improve their biocompatibility[2].
References:
[1] Rubach JK, Cui G, Schneck JL, et al. The amino-acid substituents of dipeptide substrates of cathepsin C can determine the rate-limiting steps of catalysis. Biochemistry. 2012;51(38):7551-7568.
[2] van Berkel SS, van der Lee B, van Delft FL, Wagenvoord R, Hemker HC, Rutjes FP. Fluorogenic peptide-based substrates for monitoring thrombin activity. ChemMedChem. 2012;7(4):606-617.
| Cas No. | 65147-19-5 | SDF | |
| Formula | C18H24N6O4 | M.Wt | 388.43 |
| Solubility | DMSO : 125 mg/mL (321.82 mM; Need ultrasonic); H2O : ≥ 50 mg/mL (128.73 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.5745 mL | 12.8723 mL | 25.7447 mL |
| 5 mM | 514.9 μL | 2.5745 mL | 5.1489 mL |
| 10 mM | 257.4 μL | 1.2872 mL | 2.5745 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 6 reference(s) in Google Scholar.)