Hydroxy-α-sanshool |
| Catalog No.GC36277 |
Hydroxy-α-sanshool is a transient receptor potential base 1 (TRPA1) and TRP vanilloid 1 (TRPV1) agonist with EC50 values of 69 and 1.1µM, respectively, commonly used in hyperlipidemia studies.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 83883-10-7
Sample solution is provided at 25 µL, 10mM.
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Hydroxy-α-sanshool is a transient receptor potential base 1 (TRPA1) and TRP vanilloid 1 (TRPV1) agonist with EC50 values of 69 and 1.1µM, respectively, commonly used in hyperlipidemia studies[1].
In HaCaT cells, Hydroxy-α-sanshool (10-40μM; 24h) effectively inhibited intracellular ROS production induced by C. acnes.10μM of Hydroxy-α-sanshool significantly reduced ROS levels in THP-1 cells[2]. Hydroxy-α-sanshool showed a significant protective effect on H2O2-stimulated PC12 cells without significant cytotoxicity against normal PC12 cells. Hydroxy-α-sanshool also reduced H2O2-induced apoptosis of PC12 cells by decreasing intracellular ROS and increasing mitochondrial membrane potential (MMP)[3].
Hydroxy-α-sanshool (0.5, 1.25, 2.5, 5mg/kg; po) reduces blood glucose in the T2DM mouse model by regulating glycogen metabolism through a mechanism involving the regulation of the PI3K/Akt/GSK3β/GS signaling pathway[4]. In the insulin resistance model, the Hydroxy-α-sanshool (8mg/kg) intervention lowered serum total cholesterol and LDL cholesterol and significantly reduced triacylglycerol[5].
References:
[1]. Riera C E, Menozzi‐Smarrito C, Affolter M, et al. Compounds from Sichuan and Melegueta peppers activate, covalently and non‐covalently, TRPA1 and TRPV1 channels[J]. British journal of pharmacology, 2009, 157(8): 1398-1409.
[2]. Zhou X, Su Y, Wang H, et al. Hydroxy-α-sanshool has anti-inflammatory and antioxidant effects and affects the NF-κB/STAT3 pathway via TRPV1 in acne[J]. Journal of Functional Foods, 2023, 110: 105823.
[3]. Li R L, Zhang Q, Liu J, et al. Hydroxy‐α‐sanshool Possesses Protective Potentials on H2O2‐Stimulated PC12 Cells by Suppression of Oxidative Stress‐Induced Apoptosis through Regulation of PI3K/Akt Signal Pathway[J]. Oxidative Medicine and Cellular Longevity, 2020, 2020(1): 3481758.
[4]. Zhang Q, Li R L, Wang L Y, et al. Hydroxy-α-sanshool isolated from Zanthoxylum bungeanum Maxim. has antidiabetic effects on high-fat-fed and streptozotocin-treated mice via increasing glycogen synthesis by regulation of PI3K/Akt/GSK-3β/GS signaling[J]. Frontiers in Pharmacology, 2022, 13: 1089558.
[5]. Xu F, Zhu Y, Lu M, et al. Effects of hydroxy-alpha-sanshool on intestinal metabolism in insulin-resistant mice[J]. Foods, 2022, 11(14): 2040.
| Cell experiment [1]: | |
Cell lines | PC12 cells |
Preparation Method | PC12 cells were pretreated with different concentrations of Hydroxy-α-sanshool (15, 30, and 60μM) for 2 hours and then incubated with 90μM H2O2 for another 4 hours. The control group was administered with the same amount of 1640 medium and then stimulated with H2O2. Hydroxy-α-sanshool was solubilized with DMSO and subsequently diluted in culture medium with the final concentration of DMSO less than 0.1% (v/v). |
Reaction Conditions | 15, 30, 60μM; 2h |
Applications | Hydroxy-α-sanshool showed a significant protective effect on H2O2-stimulated PC12 cells without significant cytotoxicity against normal PC12 cells. Hydroxy-α-sanshool also reduced H2O2-induced apoptosis of PC12 cells by decreasing intracellular ROS and increasing mitochondrial membrane potential (MMP). |
| Animal experiment [2]: | |
Animal models | Type 2 diabetic mouse model (T2DM) |
Preparation Method | Male C57BL/6J mice were prepared as a model of T2DM. The mice were randomly divided into five groups, including HFD/STZ (HFD feeding plus STZ treatment), HFD/STZ+Metformin (Met 150mg/kg), HFD/STZ+Hydroxy-α-sanshool -L (HAS 1.25mg/kg), HFD/STZ+HAS-M (Hydroxy-α-sanshool 2.5mg/kg), and HFD/STZ+Hydroxy-α-sanshool-H (Hydroxy-α-sanshool 5mg/kg). Subsequently, Hydroxy-α-sanshool was dissolved in 0.5% CMC-Na, and mice in the NC+Hydroxy-α-sanshool (5mg/kg) and HFD/STZ+Hydroxy-α-sanshool (1.25, 2.5, 5mg/kg) groups were administered Hydroxy-α-sanshool orally at doses of 5mg/kg, 1.25mg/kg, 2.5mg/kg, or 5mg/kg for 4 weeks, respectively. The positive control treatment used in this study was Met, which was administered daily by gavage at 150mg/kg. The mice in the NC and HFD/STZ groups received vehicle solvent. During the experiment, changes in blood glucose, body weight, feed intake, and water intake were recorded. |
Dosage form | 0.5, 1.25, 2.5, 5mg/kg; po; 4 weeks |
Applications | Hydroxy-α-sanshool reduces blood glucose in the T2DM mouse model by regulating glycogen metabolism through a mechanism involving the regulation of the PI3K/Akt/GSK3β/GS signaling pathway. |
References: | |
| Cas No. | 83883-10-7 | SDF | |
| Canonical SMILES | C/C=C/C=C/C=C\CC/C=C/C(NCC(C)(O)C)=O | ||
| Formula | C16H25NO2 | M.Wt | 263.38 |
| Solubility | DMSO : 100 mg/mL (379.68 mM; Need ultrasonic) | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.7968 mL | 18.984 mL | 37.968 mL |
| 5 mM | 0.7594 mL | 3.7968 mL | 7.5936 mL |
| 10 mM | 0.3797 mL | 1.8984 mL | 3.7968 mL |
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Quality Control & SDS
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- Purity: >98.00%
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Average Rating: 5 (Based on Reviews and 13 reference(s) in Google Scholar.)
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