N1-Acetylspermidine (hydrochloride) (Synonyms: N1-AcSPD) |
Catalog No.GC45518
One-Click Copy Product Info
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N1-Acetylspermidine (hydrochloride) is an acetyl derivative of spermidine that acts as a substrate for polyamine oxidase (PAO).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 34450-16-3
Sample solution is provided at 25 µL, 10mM.
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N1-Acetylspermidine (hydrochloride) is an acetyl derivative of spermidine that acts as a substrate for polyamine oxidase (PAO)[1]. The level of N1-Acetylspermidine in tumor tissues has significantly increased, which acts as a novel biomarker of T-lymphoblastic lymphoma/acute leukemia (T-LBL/ALL) [2]. N1-Acetylspermidine has been used to examine the interaction between DNA and polyamines during the cleavage of phosphodiester bonds at apurinic/apyrimidinic sites in DNA[3].
In vitro, N1-Acetylspermidine treatment (100μM; 8h) increased the cell migration ability of HL-60 cells[4]. Treatment with 500µM N1-Acetylspermidine for 24 hours significantly induced apoptosis in SW620 cells in the presence of apple procyanidin[5].
In vivo, N1-Acetylspermidine was injected intraperitoneally once at a dose of 30mg/kg every three days for a total of 9 days, which significantly promoted the growth of liver cancer in mice with xenografted Hepa1-6 tumors[6].
References:
[1] Takao K, Sugita Y, Shirahata A. Assay of N1-acetylpolyamine oxidase activity with N1, N11-didansylnorspermine as the substrate by ion-pair reversed phase high performance liquid chromatography[J]. Biological and Pharmaceutical Bulletin, 2010, 33(7): 1089-1094.
[2] Liu Q, Zheng W, Yang Y, et al. Metabolomic Profiling Identifies Serum N1-Acetylspermidine As a Tumor-Specific Prognostic Biomarker for T-Lymphoblastic Lymphoma/Acute Leukemia[J]. Blood, 2024, 144: 5925.
[3] Haukanes B I, Szajko K, Helland D E. Action of spermidine, N1‐acetylspermidine, and N8acetylspermidine at apurinic sites in DNA[J]. FEBS letters, 1990, 269(2): 389-393.
[4] Kato M, Maeda K, Nakahara R, et al. Acidic extracellular pH drives accumulation of N1-acetylspermidine and recruitment of protumor neutrophils[J]. PNAS nexus, 2023, 2(10): pgad306.
[5] Gossé F, Roussi S, Guyot S, et al. Potentiation of apple procyanidin-triggered apoptosis by the polyamine oxidase inactivator MDL 72527 in human colon cancer-derived metastatic cells[J]. International journal of oncology, 2006, 29(2): 423-428.
[6] Liu Z Y, Wu C Y, Wu R Q, et al. Efflux of N1-acetylspermidine from hepatoma fosters macrophage-mediated immune suppression to dampen immunotherapeutic efficacy[J]. Immunity, 2025, 58(6): 1572-1585. e10.
| Cell experiment [1]: | |
Cell lines | HL-60 cells |
Preparation Method | 1×106 HL-60 cells were inoculated into the upper chamber of the 8µm pore size transwell well. 200µl of RPMI 1640 medium containing 1% FBS was added to the upper chamber. 500µl of RPMI 1640 medium containing 10% FBS was added to the lower chamber. After culturing for 8 hours under the condition of 100µM N1-Acetylspermidine, the number of migrated cells in the lower chamber was counted. |
Reaction Conditions | 100µM; 8h |
Applications | N1-Acetylspermidine treatment increased the cell migration ability of HL-60 cells. |
| Animal experiment [2]: | |
Animal models | Wild-type C57BL/6 mice |
Preparation Method | Wild-type C57BL/6 mice (6-8 weeks old) were raised in a specific pathogen-free (SPF) environment. 1×106 Hepa1-6 cells were inoculated into the mice. 15 days later, PBS or N1-Acetylspermidine was injected intraperitoneally at a dose of 30mg/kg every 3 days for a total of 9 days. Then, the tumor volume was analyzed. |
Dosage form | 30mg/kg; every 3 days for a total of 9 days; i.p. |
Applications | N1-Acetylspermidine treatment promoted the growth of liver cancer in mice with xenografted Hepa1-6 tumors. |
References: | |
| Cas No. | 34450-16-3 | SDF | |
| Synonyms | N1-AcSPD | ||
| Canonical SMILES | CC(NCCCNCCCCN)=O.Cl.Cl | ||
| Formula | C9H21N3O.2HCl | M.Wt | 260.2 |
| Solubility | DMSO: 0.1 mg/ml,PBS (pH 7.2): 10 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.8432 mL | 19.216 mL | 38.432 mL |
| 5 mM | 768.6 μL | 3.8432 mL | 7.6864 mL |
| 10 mM | 384.3 μL | 1.9216 mL | 3.8432 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)