β-Glucan |
Catalog No.GD13990
One-Click Copy Product Info
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β-Glucan is a polysaccharide composed of D-glucose monomers linked by β-glycosidic bonds. β-Glucan has the functions of hypocholesterolemic, hypoglycemic, immunomodulatory, antitumor, antioxidant, and anti-inflammatory.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9012-72-0
Sample solution is provided at 25 µL, 10mM.
β-Glucan is a polysaccharide composed of D-glucose monomers linked by β-glycosidic bonds. β-Glucan has the functions of hypocholesterolemic, hypoglycemic, immunomodulatory, antitumor, antioxidant, and anti-inflammatory[1].
In vitro, β-Glucan (1, 2.5, 5, 10 and 20μg/mL; 24h) inhibits SVCV infection in ZF4 cells[2]. β-Glucan (1, 10, 100 and 200µg/ml; 48h) had a positive effect on increasing tumoricidal activity of macrophages[3].
In vivo, β-Glucan (0.025g/100g dry diet; 0-14 days) enhanced resistance of zebrafish against spring viraemia of carp virus (SVCV)[2]. In a mouse orthotopic pancreatic cancer model, β-Glucan (1mg/mice; once; i.p.) in combination with Irreversible electroporation (IRE) reduced local and distant tumor burden and prolonged survival[4].
References:
[1] Du B, Meenu M, Liu H, Xu B. A Concise Review on the Molecular Structure and Function Relationship of β-Glucan. Int J Mol Sci. 2019 Aug 18;20(16):4032.
[2] Liang H, Li Y, Li M, Zhou W, Chen J, Zhang Z, Yang Y, Ran C, Zhou Z. The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection. Front Immunol. 2022 Nov 3;13:1031962.
[3] Roudbary M, Daneshmand S, Hajimorad M, Roudbarmohammadip S, Hassan ZM. Immunomodulatory Effect of β-Glucan on Peritoneal Macrophages of Bab1/c Mice. Pol J Microbiol. 2015;64(2):175-9.
[4] Woeste MR, Shrestha R, Geller AE, Li S, Montoya-Durango D, Ding C, Hu X, Li H, Puckett A, Mitchell RA, Hayat T, Tan M, Li Y, McMasters KM, Martin RCG, Yan J. Irreversible electroporation augments β-glucan induced trained innate immunity for the treatment of pancreatic ductal adenocarcinoma. J Immunother Cancer. 2023 Apr;11(4):e006221.
| Cell experiment [1]: | |
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Cell lines |
ZF4 cells |
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Preparation Method |
ZF4 cells were treated with β-Glucan (1, 2.5, 5, 10, 20μg/mL) for 24h. Then 0.1 MOI SVCV was added and incubated at 22°C for 1h. Infected ZF4 cells monolayers were then washed, fresh medium added, and plates further incubated. At 24 hours after infection, SVCV replication in ZF4 cells was evaluated by qPCR measuring the expression of SVCV N protein. ZF4 cells infected with SVCV but not treated with β-Glucan is a polysaccharide composed of D-glucose monomers linked by β-glycosidic bonds. were used as control. |
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Reaction Conditions |
1, 2.5, 5, 10, 20μg/mL; 24h |
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Applications |
β-Glucan (1, 2.5, 5, 10, 20μg/mL; 24h) inhibits SVCV infection in ZF4 cells. |
| Animal experiment [2]: | |
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Animal models |
Wild-type (WT) C57Bl/6 female and male mice, 6-8 weeks old |
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Preparation Method |
On day 0, 0.1×10⁶ KPC cells were orthotopically implanted into the pancreas of the mice. On day 7 after tumor implantation, the mice were treated with a single intraperitoneal injection of β-Glucan (1mg/mouse) or PBS control. On day 14, sham surgery or IRE ablation was performed. On day 24 after tumor implantation, representative images of orthotopic pancreatic KPC tumors were obtained from the control and different treatment groups. |
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Dosage form |
1mg/mice; once; i.p. |
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Applications |
In a mouse orthotopic pancreatic cancer model, β-Glucan (1 mg/mice; once; i.p.) in combination with Irreversible electroporation (IRE) reduced local and distant tumor burden and prolonged survival. |
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References: |
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| Cas No. | 9012-72-0 | SDF | |
| Formula | M.Wt | ||
| Solubility | DMSO : 100 mg/mL (Need ultrasonic); Water：100mg/mL | Storage | Powder,-20°C,3 years;,4°C,2 years |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)