Potassium tetrachloroplatinate |
Catalog No.GD19746
One-Click Copy Product Info
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Potassium tetrachloroplatinate (K₂PtCl₄) is used as common starting material of the classic Pt(II) anticancer complexes such as cisplatin.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 10025-99-7
Sample solution is provided at 25 µL, 10mM.
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Potassium tetrachloroplatinate (K₂PtCl₄) is used as common starting material of the classic Pt(II) anticancer complexes such as cisplatin [1]. Potassium tetrachloroplatinate is used as catalyst in deuteriation of benzoic acid with D2O [2].
In vitro, Potassium tetrachloroplatinate (150μM; 1h) killed about 50% EMT6 mammary tumor cell at 37℃ [3].
In vivo, Potassium tetrachloroplatinate (10mg/kg; i.p.) induced a tumor growth delay of ~3.8 days in Lewis lung carcinoma-transplanted C57BL/6J mice, and a combination treatment with hyperthermia (43°C, 30min) did not significantly enhance the antitumor effect [3].
References:
[1] Wilson, Justin J., and Stephen J. Lippard. "Synthetic methods for the preparation of platinum anticancer complexes." Chemical reviews 114.8 (2014): 4470-4495.
[2] Kańska, Marianna, and R. Kański. "Deuteriation of benzoic acid in the presence of homogeneous potassium tetrachloroplatinate (II) catalyst." Journal of radioanalytical and nuclear chemistry 162.1 (1992): 179-186.
[3] Herman, Terence S., et al. "Effect of hyperthermia on the action of cis-diamminedichloroplatinum (ii), rhodamine 1232 [tetrachloroplatinum (ii)], rhodamine 123, and potassium tetrachloroplatinate in vitro and in vivo." Cancer research 48.9 (1988): 2335-2341.
| Cell experiment [1]: | |
Cell lines | EMT6 mammary tumor cell line |
Preparation Method | EMT6 cells were placed into T-25 flasks. Potassium tetrachloroplatinate in a small volume of sterile 0.9% phosphate-buffered saline were added to the cells in complete medium in concentrations ranging from 1 to 500μM. For hyperthermia, flasks were horizontally submersed in a Plexiglas water tank with a continuous inflow and outflow system controlled by a water temperature controller. After exposure to the agent for 1h at 37℃, 42℃, or 43℃, the cells were washed twice with saline, suspended by trypsinization, and diluted in complete growth medium and survival was measured by colony formation. The colonies were visualized by staining with crystal violet in methanol containing 2% formaldehyde. The colonies were counted manually. |
Reaction Conditions | 1-500μM; 1h |
Applications | 50% of the EMT6 were killed by about 150μM Potassium tetrachloroplatinate. At 42°C there was about a 0.4-decade enhancement in cell killing for 500μM of Potassium tetrachloroplatinate, and at 43°C there was about a 1.5-decade enhancement in cell killing at 500μM as compared to the cell killing seen at 37°C. |
| Animal experiment [1]: | |
Animal models | Lewis lung carcinoma bared male C57BL/6J mice |
Preparation Method | Potassium tetrachloroplatinate was administered intraperitoneally (i.p.) immediately prior to local hyperthermia treatment. Then immerse the tumor-bearing leg in a water bath maintained at 43°C for 30 or 60 minutes for hyperthermia apply. |
Dosage form | 10mg/kg; i.p. |
Applications | Potassium tetrachloroplatinate induced a tumor growth delay of ~3.8 days in Lewis lung carcinoma bared C57BL/6J mice, and a combination treatment with hyperthermia (43°C, 30min) did not significantly enhance the antitumor effect. |
References: | |
| Cas No. | 10025-99-7 | SDF | |
| Formula | Cl4K2Pt | M.Wt | 415.087 |
| Solubility | Storage | Store at 2-8°C, protect from light,stored under nitrogen | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4091 mL | 12.0457 mL | 24.0913 mL |
| 5 mM | 481.8 μL | 2.4091 mL | 4.8183 mL |
| 10 mM | 240.9 μL | 1.2046 mL | 2.4091 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)