Gsafe Green plus (10000× in ddH₂O)

Gum dyeing method (front dyeing method)
1. Prepare the agarose gel as usual, add concentrated 10000X Gsafe Green plus to make the final concentration in the gel 1X (for example, prepare 100ml gel, add dye 5μl-10μl, the dosage can be adjusted according to the actual situation), shake gently, pour the glue.
For Marker above 1Kb, it is recommended to reduce the dosage by half.
2. Electrophoresis in accordance with conventional methods, and observe the results.

Bubble dyeing method (post dyeing method)
1. Perform electrophoresis in accordance with conventional methods.
2. Dilute 10000X Gsafe Green plus concentrate with dH2O about 3300 times to 0.1M NaCl to make 3X staining solution. (For example, 15μl 10000X Gsafe Greenplus concentrate and 5ml 1M NaCl were added to 45ml dH2O).
3. Carefully place the gel in a suitable container and slowly add sufficient 3X staining solution to submerge the gel. Shake at room temperature for about 30 minutes. The optimal staining time depends on the gel thickness and agarose, the concentration is slightly different. For 3.5-10% acrylamide glue, the dyeing time is usually between 30min and 1 hour, then observe the results.

Notices
1.Because Gsafe Greenplus has good thermal stability, it can be added directly to the hot agarose solution without waiting for the solution to cool. Shake, oscillate or flip to make sure that the dye is thoroughly mixed. Alternatively, the Gsafe Green plus stock solution can be added to the agarose powder and the electrophoresis buffer, and then heated in a microwave oven or other common methods to prepare agarose gel. Gsafe Green plus is compatible with all commonly used electrophoresis buffer solutions.
2. If you always see band dispersion or separation is not ideal, it is recommended to use bubble dyeing to confirm whether the problem is related to the dye. If the problem persists after dyeing, it means that the problem is the dye is irrelevant, please try: reduce the concentration of agarose; choose a longer gel; extend the gel time to ensure the edge is clear; improve the sampling technique or choose the bubble staining method.
3. Gsafe Green plus has a certain affinity for glassware and non-polypropylene materials. It is recommended to use polypropylene containers in the process of dilution, storage and dyeing.
4. This method is not suitable for prefabricated polyacrylamide gels. For polyacrylamide gels, please use the bubble staining method.