HBC514 |
Catalog No.GC39621
One-Click Copy Product Info
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HBC514 is a non-fluorescent HBC analog that only emits green fluorescence upon forming a tight complex with the Pepper RNA aptamer, widely used for RNA labeling, with Ex/Em wavelengths of 485nm/530nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2530161-67-0
Sample solution is provided at 25 µL, 10mM.
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HBC514 is a non-fluorescent HBC analog that only emits green fluorescence upon forming a tight complex with the Pepper RNA aptamer, widely used for RNA labeling, with Ex/Em wavelengths of 485nm/530nm[1]. The binding between HBC514 and the Pepper aptamer exhibits high specificity, and the complex demonstrates high signal intensity and exceptional photostability in live cells, making it suitable for live-cell imaging, RNA dynamic tracking, and CRISPR-based biosensing applications[2,3].
References:
[1] CHEN X, ZHANG D, SU N, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs[J]. Nature Biotechnology, 2019, 37(11): 1287-1293.
[2] YU Q, REN K, YOU M. Genetically encoded RNA nanodevices for cellular imaging and regulation[J]. Nanoscale, 2021, 13(17): 7988-8003.
[3] YUAN D, HE H, SONG W, et al. Allosteric genetically encoded biosensor for spatiotemporal monitoring of endogenous RNA dynamics in living cells[J]. Proceedings of the National Academy of Sciences, 2025, 122(7): e2409309122.
Experimental steps for using HBC514 in live-cell imaging[1]:
(1) Cells were transfected with plasmids for 24h and then implanted into glass-bottom laser confocal culture dishes to ensure that the confluence of cells was about 60% of the next day.
(2) The cells were washed twice with 1 × PBS and then stained with Hoechst 33342 dye solution at 37℃ for 10min.
(3) After discarding the Hoechst staining solution, the cells were washed twice with 1 × PBS.Then incubated with fluorophore staining buffer (final concentration of 2μM HBC514 fluorophore diluted by the Cell Maintenance Solution, 1 × PBS containing 5% FBS) for 10min at 37℃.
(4) Microphotographs of cells were taken using the spinning disk microscope.
References:
[1] YUAN D, HE H, SONG W, et al. Allosteric genetically encoded biosensor for spatiotemporal monitoring of endogenous RNA dynamics in living cells[J]. Proceedings of the National Academy of Sciences, 2025, 122(7): e2409309122.
| Cas No. | 2530161-67-0 | SDF | |
| Canonical SMILES | CN(CCN(C)C)C(C=C1)=CC=C1/C=C(C#N)/C2=CC=C(C#N)C=C2 | ||
| Formula | C21H22N4 | M.Wt | 330.43 |
| Solubility | DMSO: 25 mg/mL (75.66 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0264 mL | 15.1318 mL | 30.2636 mL |
| 5 mM | 605.3 μL | 3.0264 mL | 6.0527 mL |
| 10 mM | 302.6 μL | 1.5132 mL | 3.0264 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 20 reference(s) in Google Scholar.)