Home>>Histone H3K27Me3 (21-44)-GK-biotin (trifluoroacetate salt)

Histone H3K27Me3 (21-44)-GK-biotin (trifluoroacetate salt) (Synonyms: ATKAAR-K(Me3)-SAPATGGVKKPHRYRPG-GK(Biotin), H3(21-44)K27me3 Substrate, Histone H3 (21-44) (Lys27me3), Lys(Me3)27-Histone H3 (21-44)-GK(Biotin))

Catalog No.GC43849

Histone H3K27Me3 (21-44)-GK-biotin is a peptide fragment of histone H3 that corresponds to amino acid residues 22-45 of the human histone H3.1 and 3.2 sequences.

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Histone H3K27Me3 (21-44)-GK-biotin (trifluoroacetate salt) Chemical Structure

Size Price Stock Qty
250μg
$255.00
In stock
1mg
$918.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Histone H3K27Me3 (21-44)-GK-biotin is a peptide fragment of histone H3 that corresponds to amino acid residues 22-45 of the human histone H3.1 and 3.2 sequences. It is trimethylated at lysine 27 and biotinylated via a C-terminal GK linker. Trimethylation of histone H3 at lysine 27 is associated with gene silencing. It is involved in tumor progression through its regulation by enhancer of zeste homolog 2 (EZH2) and transcriptional repression of tumor suppressor genes. Levels of H3K27Me3 are reduced in 293 T-REx cells containing EEDR236T and SUZ12G610V mutations and in lymphoblastoid cells isolated from patients with Weaver syndrome, a rare overgrowth disorder characterized by EZH2, EED, or SUZ12 mutations, cancer susceptibility, and various distinctive physical features. Histone H3K27Me3 (21-44)-GK-biotin has been used as a substrate for histone lysine demethylases (KDMs) to determine substrate specificity.

Chemical Properties

Cas No. SDF
Synonyms ATKAAR-K(Me3)-SAPATGGVKKPHRYRPG-GK(Biotin), H3(21-44)K27me3 Substrate, Histone H3 (21-44) (Lys27me3), Lys(Me3)27-Histone H3 (21-44)-GK(Biotin)
Formula C130H220N44O33S•XCF3COOH M.Wt 2959.5
Solubility Water: 1 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.
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