Hyaluronidase |
Catalog No.GC19789
One-Click Copy Product Info
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Hyaluronidase is an enzyme that hydrolyzes hyaluronic acid. Hyaluronidase can reduce tissue viscosity and increase tissue permeability through the hydrolysis of hyaluronic acid.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 37326-33-3;9001-54-1
Sample solution is provided at 25 µL, 10mM.
Hyaluronidase is an enzyme that hydrolyzes hyaluronic acid. Hyaluronidase can reduce tissue viscosity and increase tissue permeability through the hydrolysis of hyaluronic acid. Hyaluronidase is used in research related to drug diffusion assistance and the management of complications in medical aesthetics[1-4].
In vitro, Hyaluronidase (400μg/ml) was used to treat 3T3L1 adipocyte cell lines and mouse primary preadipocytes (from subcutaneous, visceral, or brown adipose tissue) for 8 days. Hyaluronidase significantly inhibited adipocyte differentiation and reduced lipid accumulation[5]. Hyaluronidase (400μg/ml) was applied to LiSa-2 liposarcoma cells for 24 hours. Hyaluronidase significantly increased cellular glucose uptake and lactate production, while enhancing plasma membrane GLUT1 localization and reducing TXNIP levels[6].
In vivo, Hyaluronidase (1U; 10μl) was administered via stereotactic intracranial injection in a controlled cortical impact traumatic brain injury model using C57BL/6J mice. Hyaluronidase significantly reduced brain edema after traumatic brain injury[7]. Hyaluronidase (12mg/kg) was intravenously injected into C57BL/6 mice for 30 minutes. Hyaluronidase significantly decreased the thickness of the retinal arteriolar glycocalyx and increased retinal microvascular permeability[8].
References:
[1] Yuan J, Hou B, Guo K, et al. Tumor-derived exosomal hyaluronidase 1 induced M2 macrophage polarization and promoted esophageal cancer progression. Exp Cell Res. 2024 Jun 1;439(1):113963.
[2] Shakouri A, Parvan R, Adljouy N, et al. Purification of hyaluronidase as an anticancer agent inhibiting CD44. Biomed Chromatogr. 2020 Jan;34(1):e4709.
[3] Tolwinski NS, Kano-Sueoka T. Hyaluronidase generates a single-cell suspension from cultured mouse lung epithelial cells. Biotechniques. 1997 May;22(5):856-8, 860.
[4] Meuwese MC, Broekhuizen LN, Kuikhoven M, et al. Endothelial surface layer degradation by chronic hyaluronidase infusion induces proteinuria in apolipoprotein E-deficient mice. PLoS One. 2010 Dec 8;5(12):e14262.
[5] Drygalski K, Higos R, Merabtene F, et al. Extracellular matrix hyaluronan modulates fat cell differentiation and primary cilia dynamics. Biochim Biophys Acta Mol Cell Biol Lipids. 2024 May;1869(4):159470.
[6] Sullivan WJ, Mullen PJ, Schmid EW, et al. Extracellular Matrix Remodeling Regulates Glucose Metabolism through TXNIP Destabilization. Cell. 2018 Sep 20;175(1):117-132.e21.
[7] Washington PM, Lee C, Dwyer MKR, et al. Hyaluronidase reduced edema after experimental traumatic brain injury. J Cereb Blood Flow Metab. 2020 Oct;40(10):2026-2037.
[8] Leskova W, Pickett H, Eshaq RS, et al. Effect of diabetes and hyaluronidase on the retinal endothelial glycocalyx in mice. Exp Eye Res. 2019 Feb;179:125-131.
| Cell experiment [1]: | |
Cell lines | LiSa-2 liposarcoma cells |
Preparation Method | LiSa-2 cells were treated with Hyaluronidase (400μg/ml). |
Reaction Conditions | 400μg/ml; 24h |
Applications | Hyaluronidase treatment triggered a robust increase in glycolysis, as measured by glucose uptake and lactate production. Hyaluronidase enriched GLUT1 at the plasma membrane and acutely reduced TXNIP levels. |
| Animal experiment [2]: | |
Animal models | 3 month-old male C57BL/6J mice |
Preparation Method | Mice were subjected to controlled cortical impact (CCI) traumatic brain injury. Sterile-filtered Hyaluronidase (1U) or vehicle (sterile PBS) was delivered 4min after CCI or sham injury via stereotaxic intracerebroventricular (ICV) injection. |
Dosage form | 1U in 10μl; intracerebroventricular injection; single injection |
Applications | Hyaluronidase reduced edema in the ipsilateral hippocampus at 24h after CCI compared to vehicle-treated CCI mice. Hyaluronidase reduced the percentage of hyperintense, edematous voxels in the ipsilateral hippocampus measured by T2-weighted magnetic resonance imaging. |
References: | |
| Cas No. | 37326-33-3;9001-54-1 | SDF | |
| Formula | M.Wt | ||
| Solubility | Water : 100 mg/mL (Need ultrasonic) | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Biological Activity: >300u/mg Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 33 reference(s) in Google Scholar.)