Icilin (Synonyms: AG 35) |
Catalog No.GC16882
One-Click Copy Product Info
|
TRPM8/ hENaCδ agonist
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 36945-98-9
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Icilin is an agonist of TRPM8 and hENaCδ [1, 2]
Icilin is a synthetic supercooling compound. It is reported that icilin can activate TRPM8 to some small degree in the absence of extra-cellular Ca2+. It further enhances the potency of icilin and the subsequent activation of TRPM8 channel. Icilin is also an agonist of hENaCδ. In the homomeric hENaCδ-expressing oocytes, 100μM icilin induces an inward current significantly. This effect can be reduced when the external Na+ is removed. In addition, icilin shows anti-proliferation efficacy in PC-3 cells. It induces G1 arrest via modulating the expression of cell cycle regulators including cyclin A, cyclin D1, CDK1 and CDK2. Icilin plays this role without affecting TRPM8 but through activating NK and p38 kinase pathways [1, 2 and 3]
References:
[1] Chuang H, Neuhausser W M, Julius D. The super-cooling agent icilin reveals a mechanism of coincidence detection by a temperature-sensitive TRP channel. Neuron, 2004, 43(6): 859-869.
[2] Yamamura H, Ugawa S, Ueda T, et al. Icilin activates the δ-subunit of the human epithelial Na+ channel. Molecular pharmacology, 2005, 68(4): 1142-1147.
[3] Kim S H, Kim S Y, Park E J, et al. Icilin induces G1 arrest through activating JNK and p38 kinase in a TRPM8-independent manner. Biochemical and biophysical research communications, 2011, 406(1): 30-35.
| Cas No. | 36945-98-9 | SDF | |
| Synonyms | AG 35 | ||
| Chemical Name | 3-(2-hydroxyphenyl)-6-(3-nitrophenyl)-1,4-dihydropyrimidin-2-one | ||
| Canonical SMILES | C1C=C(NC(=O)N1C2=CC=CC=C2O)C3=CC(=CC=C3)[N+](=O)[O-] | ||
| Formula | C16H13N3O4 | M.Wt | 311.29 |
| Solubility | ≥ 11.05mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.2124 mL | 16.0622 mL | 32.1244 mL |
| 5 mM | 642.5 μL | 3.2124 mL | 6.4249 mL |
| 10 mM | 321.2 μL | 1.6062 mL | 3.2124 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 25 reference(s) in Google Scholar.)