Kainic acid |
| Catalog No.: GC16667 |
Kainate receptor agonist, selective
Sample solution is provided at 25 µL, 10mM.
;)
;)
;)
;)
;)
;)
;)
;)
;)
;)
;)
;)
;)
;)
;)
;)
Quality Control & SDS
- View current batch:
-
Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
|
Animal experiment: |
The rats are fasted over night with free access to water. For stereotaxic injection, rats are anaesthetized (sodium pentobarbital, 50 mg/kg, i.p.) and immobilized in a Kopf stereotaxic frame. The rats are microinjected with 1 μg Kainic acid in 2 μL of sterilized physiological saline into the right lateral ventricle, i.e., 0.2 mm caudal from the bregma, 1.2 mm right lateral and 3.8 mm ventral according to the rat brain atlas. For control, rats are injected with same amount (2 μL) of vehicle, sterilized physiological saline. Five rats in each group are used in this study[1]. |
|
References: [1]. Matsuoka Y, et al. Kainic acid induction of heme oxygenase in vivo and in vitro. Neuroscience. 1998 Aug;85(4):1223-33. |
|
Kainic acid is a selective agonist of kainate receptor [1].
Kainate receptor is an ionotropic receptor that responds to glutamate. Presynaptic kainate receptor modulates GABA release and is involved in inhibitory neurotransmission. Postsynaptic kainate receptor is involved in excitatory neurotransmission.
In aged rats, kainic acid significantly reduced the latency to full clonic-tonic seizures and increased the amount of seizures rats. Also, kainic acid significantly increased the release of norepinephrine (NE), ASP and GLU in aged rats with clonic-tonic seizures [1]. In neonatal rats, intrahippocampal injection of kainic acid (1 μg) significantly induced pyramidal cell death [2]. In adult rats, kainic acid significantly increased the mRNA levels of neurotrophin-4/5 (NT-4/5) in the dorsal horn and in the spinal cord white matter, and increased the mRNA level of brain-derived neurotrophic factor (BDNF) in the ventral horn. While kainic acid didn’t affect neurotrophin-3 (NT-3). These results suggested that NT-4/5 and BDNF participated in the response of the spinal cord to excitotoxic stimuli induced by kainic acid [3].
References:
[1]. Dawson R Jr, Wallace DR. Kainic acid-induced seizures in aged rats: neurochemical correlates. Brain Res Bull, 1992, 29(3-4): 459-468.
[2]. Cook TM, Crutcher KA. Intrahippocampal injection of kainic acid produces significant pyramidal cell loss in neonatal rats. Neuroscience, 1986, 18(1): 79-92.
[3]. Scarisbrick IA, Isackson PJ, Windebank AJ. Differential expression of brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4/5 in the adult rat spinal cord: regulation by the glutamate receptor agonist kainic acid. J Neurosci, 1999, 19(18): 7757-7769.
| Cas No. | 487-79-6 | SDF | |
| Chemical Name | (2S,3S,4S)-3-(carboxymethyl)-4-(prop-1-en-2-yl)pyrrolidine-2-carboxylic acid | ||
| Canonical SMILES | OC([C@H]1NC[C@H](C(C)=C)[C@@H]1CC(O)=O)=O | ||
| Formula | C10H15NO4 | M.Wt | 213.23 |
| Solubility | ≥ 11.05mg/mL in Water with gentle warming | Storage | Store at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
| Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5 (Based on Reviews and 25 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *