Kinetin (Synonyms: N6-furfuryladenine, NSC 23119) |
Catalog No.GC11023
One-Click Copy Product Info
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Kinetin is a plant hormone that can regulate gene expression, cell cycle and calcium flux.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 525-79-1
Sample solution is provided at 25 µL, 10mM.
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Kinetin is a plant hormone that can regulate gene expression, cell cycle and calcium flux [1]. Kinetin can regulate DNA repair and proteosome-mediated protein turnover, and activate Arabidopsis cell division through induction of the d-type cyclin CycD3 [2]. Kinetin has been widely used to protects DNA against oxidative damage to 8-oxo-dG mediated by the Fenton reaction [3].
In vitro, Kinetin treatment (10μM) for 7 days significantly stimulated differentiation of C2C12 cells into myotubes [4]. Treatment with 40μM Kinetin for 2 days significantly inhibited the growth of keratinocytes and increased the levels of the Involucrin and K10 protein [5]. The 24-hour treatment with 500μM Kinetin significantly inhibited the viability of HL-60 cells[6].
In vivo, Kinetin treatment via oral administration at a dose of 20mg/kg daily for 90 days significantly improved cognitive impairment in mice induced by aluminum chloride and D-galactose, and reduced brain oxidative stress[7]. Daily intraperitoneal injection of 1mg/kg dose of Kinetin for 10 days significantly alleviated the oxidative stress in the liver of rats induced by cisplatin and reduced the levels of CRP, IL-6 and CD95[8].
References:
[1] Barciszewski J, Siboska G, Rattan S I S, et al. Occurrence, biosynthesis and properties of kinetin (N6-furfuryladenine)[J]. Plant growth regulation, 2000, 32(2): 257-265.
[2] Barciszewski J, Rattan S I S, Siboska G, et al. Kinetin—45 years on[J]. Plant science, 1999, 148(1): 37-45.
[3] Barciszewski J, Massino F, Clark B F C. Kinetin—a multiactive molecule[J]. International Journal of Biological Macromolecules, 2007, 40(3): 182-192.
[4] Mielcarek M, Isalan M. Kinetin stimulates differentiation of C2C12 myoblasts[J]. PLoS One, 2021, 16(10): e0258419.
[5] Berge U, Kristensen P, Rattan S I S. Kinetin‐induced differentiation of normal human keratinocytes undergoing aging in vitro[J]. Annals of the New York Academy of Sciences, 2006, 1067(1): 332-336.
[6] Naseem M, Othman E M, Fathy M, et al. Integrated structural and functional analysis of the protective effects of kinetin against oxidative stress in mammalian cellular systems[J]. Scientific reports, 2020, 10(1): 13330.
[7] Wei Y, Liu D, Zheng Y, et al. Protective effects of kinetin against aluminum chloride and D-galactose induced cognitive impairment and oxidative damage in mouse[J]. Brain research bulletin, 2017, 134: 262-272.
[8] Fathy M, Darwish M A, Abdelhamid A S M, et al. Kinetin ameliorates cisplatin-induced hepatotoxicity and lymphotoxicity via attenuating oxidative damage, cell apoptosis and inflammation in rats[J]. Biomedicines, 2022, 10(7): 1620.
| Cell experiment [1]: | |
Cell lines | HL-60 cells |
Preparation Method | HL-60 cells were grown in RPMI-1640 medium with 10% (v/v) fetal bovine serum (FBS), 1% (w/v) l-glutamine and 0.4% (w/v) antibiotics (50U/ml penicillin, and 50mg/ml streptomycin) at 37°C in 5% CO2/atmosphere. Cells were seeded at a density of 3.5×105 cells/ml in the 6-well plates and allowed to adhere in a 5% CO2 incubator at 37°C for 24h. The cells were treated with different concentrations of Kinetin (0, 0.5, 1, 10, 100, and 500μM) for 24h. The cell viability was evaluated. |
Reaction Conditions | 0, 0.5, 1, 10, 100, and 500μM; 24h |
Applications | Kinetin treatment significantly reduced cell viability of HL-60 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Kunming mice |
Preparation Method | Kunming mice (18-22g) were housed singly in a standard environment with food and water ad libitum. After a one-week adaptation period, the mice were administered 20mg/kg of Kinetin orally daily, drank water containing 10mg/kg of aluminum chloride, and were given subcutaneous injections of D-galactose (100mg/kg/day) for 90 days. Then, the mouse brain tissues were collected for analysis. |
Dosage form | 20mg/kg/day; 90 days; p.o. |
Applications | Kinetin treatment significantly attenuated the histopathological changes in hippocampus CA3 region of mouse brain tissues. |
References: | |
| Cas No. | 525-79-1 | SDF | |
| Synonyms | N6-furfuryladenine, NSC 23119 | ||
| Chemical Name | N-(furan-2-ylmethyl)-7H-purin-6-amine | ||
| Canonical SMILES | C1=COC(=C1)CNC2=NC=NC3=C2NC=N3 | ||
| Formula | C10H9N5O | M.Wt | 215.21 |
| Solubility | ≥ 5.38 mg/mL in DMSO with gentle warming | Storage | Store at-20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.6466 mL | 23.2331 mL | 46.4662 mL |
| 5 mM | 929.3 μL | 4.6466 mL | 9.2932 mL |
| 10 mM | 464.7 μL | 2.3233 mL | 4.6466 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)