Lalistat 2 |
Catalog No.GC44031
One-Click Copy Product Info
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Lalistat 2 is an inhibitor of lysosomal acid lipase (LAL), with an IC50 value of 152nM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1234569-09-5
Sample solution is provided at 25 µL, 10mM.
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Lalistat 2 is an inhibitor of lysosomal acid lipase (LAL), with an IC50 value of 152nM[1]. Lalistat 2, by targeting and inhibiting LAL, will disrupt the function of lysosomes and promote the formation of the pro-inflammatory adipose tissue macrophage (ATM) phenotype[2]. Lalistat 2 has been widely used to specifically measure LAL activity in human blood samples and cells [3].
In vitro, Lalistat 2 treatment at 0.1μM for 4 days led to lipid accumulation in proliferating C2C12 cells, with significant increases in triglyceride and total cholesterol levels[4]. Treatment with 100μM of Lalistat 2 for 7 days led to alterations in the lipid metabolism of mouse bone marrow stromal cells (BMSCs) and impaired cell differentiation[5]. Lalistat 2 treatment (30μΜ; 24h) inhibited the lipase of triglycerides and hormone-sensitive lipase in COS-7 cells[6]. Treatment with 10μM of Lalistat 2 for 6 hours reduced the release of lipid mediators in peritoneal macrophages[7].
References:
[1] Rosenbaum A I, Cosner C C, Mariani C J, et al. Thiadiazole carbamates: potent inhibitors of lysosomal acid lipase and potential Niemann− Pick type C disease therapeutics[J]. Journal of medicinal chemistry, 2010, 53(14): 5281-5289.
[2] Wehr R, Lindhorst A, Arndt L, et al. Lysosomal exocytosis by macrophages as a druggable mechanism for anti-inflammatory clearance of dead adipocytes in adipose tissue[J]. Cell Death & Disease, 2025.
[3] Hamilton J, Jones I, Srivastava R, et al. A new method for the measurement of lysosomal acid lipase in dried blood spots using the inhibitor Lalistat 2[J]. Clinica chimica acta, 2012, 413(15-16): 1207-1210.
[4] Akhmetshina A, Schooltink L, Amor M, et al. Loss or inhibition of lysosomal acid lipase in vitro leads to cholesteryl ester accumulation without affecting muscle formation or mitochondrial function[J]. BBA advances, 2025, 7: 100135.
[5] Helderman R C, Whitney D G, Duta-Mare M, et al. Loss of function of lysosomal acid lipase (LAL) profoundly impacts osteoblastogenesis and increases fracture risk in humans[J]. Bone, 2021, 148: 115946.
[6] Bradić I, Kuentzel K B, Honeder S, et al. Off-target effects of the lysosomal acid lipase inhibitors Lalistat-1 and Lalistat-2 on neutral lipid hydrolases[J]. Molecular Metabolism, 2022, 61: 101510.
[7] Schlager S, Vujic N, Korbelius M, et al. Lysosomal lipid hydrolysis provides substrates for lipid mediator synthesis in murine macrophages[J]. Oncotarget, 2017, 8(25): 40037.
| Cell experiment [1]: | |
Cell lines | C2C12 cells |
Preparation Method | The C2C12 cells were cultured in high-glucose DMEM medium, supplemented with 10% low-fat serum (LPDS) and 1% penicillin/streptomycin, and were incubated in a 37°C, 5% CO2 incubator. The cells were cultured with 0.1μM of Lalistat 2 for 4 days, and then the lipid levels within the cells were analyzed. |
Reaction Conditions | 0.1µM; 4 days |
Applications | Lalistat 2 treatment resulted in lipid accumulation in the C2C12 cells. |
References: | |
| Cas No. | 1234569-09-5 | SDF | |
| Canonical SMILES | O=C(N1CCCCC1)OC2=NSN=C2N3CCCCC3 | ||
| Formula | C13H20N4O2S | M.Wt | 296.4 |
| Solubility | DMF: 20 mg/ml,DMSO: 20 mg/ml,Ethanol: 20 mg/ml,Ethanol:PBS(pH 7.2) (1:5): 0.16 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3738 mL | 16.8691 mL | 33.7382 mL |
| 5 mM | 674.8 μL | 3.3738 mL | 6.7476 mL |
| 10 mM | 337.4 μL | 1.6869 mL | 3.3738 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)