GMax™ Liposome2000 Transfection Reagent
Sample solution is provided at 25 µL, 10mM.
- View current batch:
Preparation Before Experiment
1.Plate cells so they will be 70–90% confluent at the time of transfection.
2.Prepare plasmid DNA-lipid complexes.
3.Add DNA-lipid complexes to cells.
A. GMax™ Liposome2000 DNA Transfection Reagent Protocol
|Final DNA per well||100 ng||500 ng||2500 ng|
|Final GMax™ Liposome2000 Reagent per well||0.2–0.5 μL||1.0–2.5 μL||5.0–12.5 μL|
B. Co-Transfection of Plasmid DNA and siRNA
Transfect plasmid DNA and siRNA at the same time using GMax™ Liposome2000 Reagent by adding 30 pmol (~0.6 μg) of siRNA per 1 μg of DNA.
C. mRNA Transfection
mRNA can be transfected in a 24-well plate using GMax™ Liposome2000 Reagent by adding 0.5–1 μg of mRNA per well.
D. GMax™ Liposome2000 DNA Transfection Reagent Protocol
Transfect cells according to the following chart. Volumes are given on a per-well basis. Each reaction mix is sufficient for triplicate (96-well), duplicate (24-well), and single well (6-well) transfections, and accounts for pipetting variations.
GMax™ Liposome2000 Transfection Reagent is a proprietary formulation for transfecting nucleic acids into a wide range of eukaryotic cells. DNA-GMax™ Liposome2000 complexes must be made in serum-free medium such as Opti-MEM® Reduced Serum Medium and can be added directly to cells in culture medium, in the presence or absence of serum/antibiotic. It is not necessary to remove complexes or change/add medium after transfection. The amount of GMax™ Liposome2000 Reagent required for successful transfection varies depending on the cell type and passage number. Start any new transfection by testing the recommended four concentrations of GMax™ Liposome2000 Reagent to determine an optimum amount.
|Components||0.75 mL||1.5 mL|
|GMax™ Liposome2000 Transfection Reagent||0.75 mL||0.75 mL * 2|
|Store at 2–8°C. Do not freeze.|