Malvidin-3-galactoside chloride |
Catalog No.GC63058
One-Click Copy Product Info
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Malvidin-3-galactoside chloride is an anthocyanin monomer that possesses antioxidant activity.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 30113-37-2
Sample solution is provided at 25 µL, 10mM.
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Malvidin-3-galactoside chloride is an anthocyanin monomer that possesses antioxidant activity[1-2]. Malvidin-3-galactoside chloride is used in research related to cancer, cardiovascular diseases, and intestinal microbiota regulation[3-4].
In vitro, human retinal capillary endothelial cells (HRCECs) were pretreated with Malvidin-3-galactoside chloride (10µg/mL) for 24 hours, followed by stimulation with high glucose (30mmol/L) for 24-48 hours. Malvidin-3-galactoside chloride significantly reduced reactive oxygen species levels, increased the activity of antioxidant enzymes (CAT, SOD), downregulated Nox4 expression, altered nitric oxide levels, while also inhibiting vascular endothelial growth factor production and lowering intercellular adhesion molecule-1 levels, thereby exerting antioxidant and anti-inflammatory effects to protect the cells[5]. Human umbilical vein endothelial cells (HUVECs) were pretreated with Malvidin-3-galactoside chloride (1–100µM) for 18 hours, followed by stimulation with TNF-α (10µg/L) for 6 hours. Malvidin-3-galactoside chloride significantly and concentration-dependently inhibited the expression of monocyte chemoattractant protein-1, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1 at both the protein and mRNA levels, while also inhibiting IκBα degradation and blocking the nuclear translocation of the p65 protein[6].
In vivo, C57BL/6J male mice subcutaneously injected with human hepatocellular carcinoma HepG2 cells were administered Malvidin-3-galactoside chloride (40mg/kg or 80mg/kg) via oral gavage every other day for 4 consecutive weeks. Malvidin-3-galactoside chloride significantly inhibited tumor growth, promoted tumor cell apoptosis, inhibited cell proliferation, and upregulated PTEN expression while downregulating p-AKT expression in the tumor tissues[7].
References:
[1] Jie Lin, Jinlong Tian, Chi Shu, et al. Malvidin-3-galactoside from blueberry suppresses the growth and metastasis potential of hepatocellular carcinoma cell Huh-7 by regulating apoptosis and metastases pathway. Food Science and Human Wellness. 2020;9(2):136-145.
[2] Rutledge GA, Sandhu AK, Miller MG, et al. Blueberry phenolics are associated with cognitive enhancement in supplemented healthy older adults. Food Funct. 2021 Jan 7;12(1):107-118.
[3] Huang WY, Wu H, Li DJ, et al. Protective Effects of Blueberry Anthocyanins against H2O2-Induced Oxidative Injuries in Human Retinal Pigment Epithelial Cells. J Agric Food Chem. 2018 Feb 21;66(7):1638-1648.
[4] Huang W, Zhu Y, Li C, et al. Effect of Blueberry Anthocyanins Malvidin and Glycosides on the Antioxidant Properties in Endothelial Cells. Oxid Med Cell Longev. 2016;2016:1591803.
[5] Huang W, Yan Z, Li D, et al. Antioxidant and Anti-Inflammatory Effects of Blueberry Anthocyanins on High Glucose-Induced Human Retinal Capillary Endothelial Cells. Oxid Med Cell Longev. 2018 Feb 22;2018:1862462.
[6] Huang WY, Liu YM, Wang J, et al. Anti-inflammatory effect of the blueberry anthocyanins malvidin-3-glucoside and malvidin-3-galactoside in endothelial cells. Molecules. 2014 Aug 21;19(8):12827-41.
[7] Wang Y, Lin J, Tian J, et al. Blueberry Malvidin-3-galactoside Suppresses Hepatocellular Carcinoma by Regulating Apoptosis, Proliferation, and Metastasis Pathways In Vivo and In Vitro. J Agric Food Chem. 2019 Jan 16;67(2):625-636.
| Cell experiment [1]: | |
Cell lines | Human umbilical vein endothelial cells (HUVECs) |
Preparation Method | HUVECs were maintained in M199 medium supplemented with fetal bovine serum. Cells were pretreated with Malvidin-3-galactoside chloride (1, 10, 50, and 100µM) for 18 hours, followed by stimulation with Tumor Necrosis Factor-alpha (TNF-α; 10µg/L) for 6 hours. |
Reaction Conditions | 1-100µM; 18h pretreatment |
Applications | Malvidin-3-galactoside chloride inhibited TNF-α-induced increases in the production of monocyte chemotactic protein-1 (MCP-1), intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) in a concentration-dependent manner, both at the protein and mRNA levels. Malvidin-3-galactoside chloride also decreased IκBα degradation and blocked the nuclear translocation of the p65 subunit, indicating its anti-inflammatory mechanism is mediated by blocking the Nuclear Factor-kappa B (NF-κB) pathway. |
| Animal experiment [2]: | |
Animal models | C57BL/6 mice |
Preparation Method | Mice were injected with 1 x 10⁶ HepG2 cells. Starting 1 week after injection, mice were administered Malvidin-3-galactoside chloride (40mg/kg or 80mg/kg) by gavage every other day for 4 consecutive weeks. A control group received distilled water. |
Dosage form | 40mg/kg or 80mg/kg; Oral gavage; Every other day for 4 weeks. |
Applications | Malvidin-3-galactoside chloride administration, particularly at 80mg/kg, significantly inhibited tumor growth, promoted tumor cell apoptosis, and inhibited cell proliferation. Malvidin-3-galactoside chloride also upregulated PTEN expression and downregulated p-AKT expression in tumor tissues. |
References: | |
| Cas No. | 30113-37-2 | SDF | |
| Formula | C23H25ClO12 | M.Wt | 528.89 |
| Solubility | Storage | Store at 2-8°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8908 mL | 9.4538 mL | 18.9075 mL |
| 5 mM | 378.2 μL | 1.8908 mL | 3.7815 mL |
| 10 mM | 189.1 μL | 945.4 μL | 1.8908 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 16 reference(s) in Google Scholar.)