MHI-148 |
Catalog No.GC68203
One-Click Copy Product Info
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MHI-148 is a near-infrared heptamethine cyanine dye with tumor-targeting properties (excitation/emission wavelengths: 740/950nm).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 172971-76-5
Sample solution is provided at 25 µL, 10mM.
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MHI-148 is a near-infrared heptamethine cyanine dye with tumor-targeting properties (excitation/emission wavelengths: 740/950nm). MHI-148 can be specifically taken up and accumulated in the lysosomes and mitochondria of tumor cells via a hypoxia-mediated mechanism. MHI-148 can be used for research related to tumor detection, diagnosis, and photodynamic therapy[1-4].
References:
[1] Thomas RG, Moon MJ, Surendran SP, et al. MHI-148 Cyanine Dye Conjugated Chitosan Nanomicelle with NIR Light-Trigger Release Property as Cancer Targeting Theranostic Agent. Mol Imaging Biol. 2018 Aug;20(4):533-543.
[2] Choi PJ, Tomek P, Tercel M, et al. Conjugation of Palbociclib with MHI-148 Has an Increased Cytotoxic Effect for Breast Cancer Cells and an Altered Mechanism of Action. Molecules. 2022 Jan 27;27(3):880.
[3] Usama SM, Jiang Z, Pflug K, et al. Conjugation of Dasatinib with MHI-148 Has a Significant Advantageous Effect in Viability Assays for Glioblastoma Cells. ChemMedChem. 2019 Sep 4;14(17):1575-1579.
[4] Raveendran A, Poilil Surendran S, Ser J, et al. Heptamethine Cyanine Dye MHI-148-Mediated Drug Delivery System to Enhance the Anticancer Efficiency of Paclitaxel. Int J Nanomedicine. 2021 Oct 21;16:7169-7180.
Application of MHI-148 for Near-Infrared Fluorescence Imaging in a Lung Cancer Model[1]
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
1. Reagent Preparation: MHI-148 is dissolved in PBS buffer to prepare a solution with a concentration of 7.5µM.
2. Cell and Animal Model Preparation: A549 cells are routinely subcultured in RPMI-1640 medium supplemented with 10% fetal bovine serum under conditions of 37°C and 5% CO₂. An A549 cell suspension (5×10⁶ cells) is subcutaneously injected into the right flank of nude mice. The tumors are allowed to grow for 6-8 weeks until reaching approximately 0.8cm in diameter before being used for imaging experiments.
3. In Vivo Fluorescence Imaging: MHI-148 injection (dose: 1.5nmol/mouse) is administered intravenously via the tail vein to tumor-bearing mice. In vivo imaging is performed using a small animal imaging system (excitation/emission wavelengths: 740/950nm). The tumor-to-background signal ratio (TBR) is calculated using muscle tissue from the contralateral side at the same position as the background.
4. Ex Vivo Fluorescence Imaging: Mice are sacrificed at 2, 6, 24, 48, and 96 hours after MHI-148 injection, and major organs (tumor, heart, lung, liver, spleen, kidney, intestine, muscle, bone) are harvested. The fluorescence intensity of each tissue is measured using the same imaging system, and the signal ratio of tumor to muscle is calculated.
Precautions:
(1) MHI-148 is water-soluble, primarily cleared from the body via the liver and intestine, exhibits low residual fluorescence in non-target organs, and provides a high signal-to-noise ratio.
(2) Please comply with animal experiment ethics guidelines and obtain approval from the relevant Institutional Animal Care and Use Committee.
(3) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:
[1] Xia X, Gai Y, Feng H, et al. Florescence Imaging Lung Cancer with a Small Molecule MHI-148. J Fluoresc. 2020 Dec;30(6):1523-1530.
| Cas No. | 172971-76-5 | SDF | |
| Formula | C42H52BrClN2O4 | M.Wt | 764.23 |
| Solubility | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.3085 mL | 6.5425 mL | 13.0851 mL |
| 5 mM | 261.7 μL | 1.3085 mL | 2.617 mL |
| 10 mM | 130.9 μL | 654.3 μL | 1.3085 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)