Mito-TEMPO |
| Catalog No.GC31682 |
Mito-Tempo is a mitochondria-targeted antioxidant with effective superoxide scavenging properties, which converts toxic superoxide molecules into hydrogen peroxide or oxygen and subsequently detoxified to oxygen and water by catalase or glutathione peroxidase.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1334850-99-5
Sample solution is provided at 25 µL, 10mM.
Mito-Tempo is a mitochondria-targeted antioxidant with effective superoxide scavenging properties, which converts toxic superoxide molecules into hydrogen peroxide or oxygen and subsequently detoxified to oxygen and water by catalase or glutathione peroxidase[1].
In vitro, Mito-Tempo has a greater protective effect by enhancing superoxide dismutase (SOD) activity and PI3K/AKT/mTOR phosphorylation. Glutamate-exposed cells significantly increased cellular oxidative stress by enhancing ROS production. Glutamate treatment also increased LDH release following the loss of mitochondrial membrane potential, causing cell viability loss. Treatment with Mito-Tempo not only attenuated the generation of ROS and improved mitochondrial membrane potential but also reduced the neurotoxicity of glutamate in a concentration-dependent manner, which leads to increased cell viability and decreased LDH release[2].
In vivo, Mito-TEMPO pretreatment inhibited inflammation, attenuated LPS-induced liver injury, and enhanced the antioxidative capability in septic mice, as evidenced by the decreased MDA content and the increased SOD activity. In addition, Mito-TEMPO restored mitochondrial size and improved mitochondrial function. Finally, we found that the levels of pyroptosis-related proteins in the liver of LPS-treated mice were lower after pretreatment with Mito-TEMPO [3].
References:
[1]. Liang HL, Sedlic F, et al. SOD1 and MitoTEMPO partially prevent mitochondrial permeability transition pore opening, necrosis, and mitochondrial apoptosis after ATP depletion recovery. Free Radic Biol Med. 2010 Nov 30;49(10):1550-60.
[2]. Mukem S, Thongbuakaew T, et al. Mito-Tempo suppresses autophagic flux via the PI3K/Akt/mTOR signaling pathway in neuroblastoma SH-SY5Y cells. Heliyon. 2021 Jun 15;7(6):e07310.
[3]. Mukem S, Thongbuakaew T, et al. Mito-Tempo suppresses autophagic flux via the PI3K/Akt/mTOR signaling pathway in neuroblastoma SH-SY5Y cells. Heliyon. 2021 Jun 15;7(6):e07310.
| Cell experiment [1]: | |
Cell lines | Human neuroblastoma cells (SH-SY5Y ) |
Preparation Method | SH-SY5Y cells were treated with 100 μM glutamate in the presence or absence of Mito-Tempo for 24 h. The cell viability was assessed by the mitochondrial performance, using the MTT reduction assay. |
Reaction Conditions | 50, 100 µM Mito-Tempo for 24h. |
Applications | 10 and 100 μM glutamate significantly reduced the number of the living cell to 88.86 ± 3.45% and 51.12 ± 2.97%. 50 and 100 μM Mito-Tempo significantly restored cell viability to 82.90 ± 1.78% and 93.56 ± 2.85%, respectively, indicating that Mito-Tempo could protect the cells from glutamate toxicity. |
| Animal experiment [2]: | |
Animal models | C57BL/6 mice |
Preparation Method | Lipopolysaccharide- (LPS-) induced experimental sepsis mouse model was established by injecting male mice intraperitoneally with LPS. Mito-TEMPO was intraperitoneally injected 1 h prior to LPS injection. |
Dosage form | 20 mg/kg Mito-TEMPO, intraperitoneal(i.p.) injection |
Applications | In the LPS+Mito-TEMPO group, the levels of alanine transaminase(ALT) and aspartate transaminase (AST) were approximately 1.5- and 2-fold lower compared with those in the LPS group. Mito-TEMPO pretreatment protects mice against LPS-Induced acute liver injury. |
References: | |
| Cas No. | 1334850-99-5 | SDF | |
| Canonical SMILES | [O]N1C(C)(C)CC(NC(C[P+](C2=CC=CC=C2)(C3=CC=CC=C3)C4=CC=CC=C4)=O)CC1(C)C.[Cl-] | ||
| Formula | C29H35ClN2O2P | M.Wt | 510.03 |
| Solubility | DMSO : 125 mg/mL (245.08 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9607 mL | 9.8033 mL | 19.6067 mL |
| 5 mM | 0.3921 mL | 1.9607 mL | 3.9213 mL |
| 10 mM | 0.1961 mL | 0.9803 mL | 1.9607 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >98.00%
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Related Biological Data
TM3 cells were pretreated with 10 μM M-T for 4 h, and then incubated with 10 μM GLY for another 24 h to carry out the indicated assays.Representative confocal images of LC3 (green) colocalizationwith TOM 20 (Red) by confocal microscopy and quantified analysis in three batches of cells with 50 cells per group in each independent experiment.
TM3 cells were pretreated with 10 μM M-T（GlpBio) for 4 h, and then incubated with 10 μM GLY for another 24 h to carry out the indicated assays.
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Arabinose confers protection against DSS-induced epithelial tight junction injury by regulation of mitochondrial function.Representative western blotting results showing the expression of ZO-1, occludin, claudin-1 and claudin-4 protein levels in the DSS-treated cells in response to 5 μM retenone or 2 μM mito-TEMPO challenge.
The mito-TEMPO (2μM, GlpBio, GC31682), a mitochondrial-targeted antioxidant, was used to stimulate cells along with DSS plus arabinose stimulation.
International Immunopharmacology, 2024, 126: 111188. PMID: 37995573 IF: 5.5999 -
Related Biological Data
Mito-TEMPO rescued the suppressive effect of LIPUS on GSC stem cell properties.O2 generation in GSCs. The results of quantified fluorescence images are normalized to Ctrl.
MitoTEMPO (5 μM GlpBio) inhibited mitochondrial O2 generation after LIPUS treatment. O2 generation analysis in GSCs 24 h after treatment with LIPUS and mito-TEMPO (5 μM) using the microplate reader.
Iscience 25.1 (2022): 103558. IF: 5.451
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