Monochlorobimane (Chlorobimane) (Synonyms: Chlorobimane) |
| Catalog No.GC30550 |
Monochlorobimane (Chlorobimane) (Chlorobimane) is a fluorescent dye (Λex=380 nm, Λem=470 nm) to measure glutathione (GSH) in cellular assays.
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Cas No.: 76421-73-3
Sample solution is provided at 25 µL, 10mM.
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Monochlorobimane is a fluorescent dye (λex=380 nm, λem=470 nm).
A common technique to measure Glutathione (GSH) in cultured cells is to add Monochlorobimane to the culture medium where it readily enters cells to form a fluorescent GSH-monochlorobimane adduct that can be measured fluorometrically. It is clear that the Monochlorobimane approach gives comparable results to the HPLC technique. Although most laboratories use 100 mM Monochlorobimane in their assay our results show that the Monochlorobimane concentration can be lowered to 25 mM and still accurately measure homogenate GSH content[1].
[1]. Kamencic H, et al. Monochlorobimane fluorometric method to measure tissue glutathione. Anal Biochem. 2000 Nov 1;286(1):35-7.
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