Protease Inhibitor Cocktail (EDTA-Free,100× in DMSO) |
| Catalog No.: GK10014 |
Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability.
Sample solution is provided at 25 µL, 10mM.
- ACS Infect Dis (2021).PMID:33723986
- Front Pharmacol 12 (2021).PMID:34880751
- Cell Death Dis 12.12 (2021)1-12.PMID:34862372
- Arch Toxicol (2022)1-15.
- Bioengineered 13.4 (2022)9197-9210.PMID:35383533
- Behav Brain Res (2022)114002.PMID:35810999
- J Neuroinflamm (2022)1-17.PMID:35705998
- Int Immunopharmacol 116 (2023)109637.
- Iscience(2023)
Related Biological Data
- View current batch:
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts), before assaying.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability.
Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of inhibitors of those enzymes known to be present.
Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.
Protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. This cocktail is supplied as a ready-to-use solution in DMSO.
| Ingredient | M.W. | 100x Conc. | Target |
| AEBSF | 239.5 | 104 mM | Serine proteases |
| Aprotinin | 6511.5 | 80 μM | Serine proteases |
| Bestatin | 308.38 | 5 mM | Aminopeptidases |
| E-64 | 357.4 | 1.5 mM | Cysteine proteases |
| Leupeptin | 475.6 | 2 mM | Alkaline phosphatases |
| Pepstatin A | 685.9 | 1.5 mM | Aspartic proteases |
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *