Protease Inhibitor Cocktail (EDTA-Free,100× in DMSO) |
| Catalog No.GK10014 |
Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
- Cell Commun Signal 22.1 (2024)：1-24.PMID:38610001
- Inflamm Res 72.9 (2023)：1773-1787.PMID:37659014
- Cell Rep 43.8 (2024).PMID:39106181
- Food Funct (2024).PMID:38602356
- Front Pharmacol 12 (2021)：735194.PMID:34880751
- Int Immunopharmacol 130 (2024)：111750.PMID:38442577
- Int Immunopharmacol 130 (2024)：111811.PMID:38457929
- Mol Med 30.1 (2024)：1-14.PMID:38760717
- Neural Regen Res 18.12 (2023)：2711-2719.PMID:37449635
- Mol Med 30.1 (2024)1-18.PMID:39138413
- Front Pharmacol 13 (2022)：938134.PMID:36210836
- Front Cell Infect Mi 12 (2022)：877265.PMID:35444960
- Cell Mol Life Sci 81.1 (2024)：459.PMID:39562370
- Cell Death Discov 11.1 (2025)：46.PMID:39905004
- Biochem Pharmacol (2024)：116230.PMID:38643905
- Skeletal Muscle 14 (2024).PMID:39026344
- Geroscience (2024)：1-18.PMID:38922380
- Acs Infect Dis 10.3 (2024)：988-999.PMID:38317607
- ACS Infect Dis 7.4 (2021)：777-789.PMID:33723986
- Drug Des Dev Ther (2025)：1681-1701.
- J Ethnopharmacol (2023)：117537.PMID:38043756
- Acs Infect Dis (2024).PMID:38317607
- Front Pharmacol 15 (2024)：1477122.PMID:39697555
- Acta Bioch Bioph Sin 55.10 (2023)：1561.PMID:37635409
- Biomolecules and Biomedicine (2024).PMID:38958450
- Biomol Biomed (2024).PMID:38958450
- Acta Bioch Bioph Sin 56.7 (2024)：1022.PMID:38818581
- Mol Med Rep 30.1 (2024)：1-11.PMID:38757346
- Behav Brain Res 433 (2022)：114002.PMID:35810999
- Bioengineered 13.4 (2022)：9197-9210.PMID:35383533
- J Pharm Pharmacol (2024)：rgae060.PMID:38767973
- Acta Crystallogr F (2024).PMID:38818823
- Adv Funct Mater (2024)：2305827.
- J Control Release 372 (2024)：778-794.PMID:38936744
- Redox Biol 65 (2023)：102824.PMID:37517320
- Iscience 26.4 (2023).PMID:37035004
- Int Immunopharmacol 116 (2023)：109637.PMID:36764283
- Arch Toxicol 96.3 (2022)：767-781.PMID:35088107
Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability.
Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of inhibitors of those enzymes known to be present.
Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.
Protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. This cocktail is supplied as a ready-to-use solution in DMSO.
| Ingredient | Summary | CAS Number | Targets |
| Aprotinin | Inhibitor of bovine pancreatic trypsin | 9087-70-1 | Proteases|Serine Protease |
| Bestatin | Aminopeptidase inhibitor | 58970-76-6 | Proteases|Aminopeptidase |
| E-64 | Cysteine protease inhibitor,irriversible | 66701-25-5 | Proteases|Cathepsin |
| Leupeptin, Microbial | Inhibitor of serine and cysteine proteases | 103476-89-7 | Proteases|Serine Protease |
| Pepstatin A | Aspartic proteinases inhibitor | 26305-03-3 | Proteases|Other Proteases |
| AEBSF.HCl | Serine protease inhibitor | 30827-99-7 | Proteases|Serine Protease |
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts), before assaying.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
- View current batch:
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *