SNAP 398299 |
Catalog No.GC50161
One-Click Copy Product Info
|
SNAP 398299 is a selective antagonist of the galanin-3 receptor (Gal3).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 903878-06-8
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
SNAP 398299 is a selective antagonist of the galanin-3 receptor (Gal3)[1-2]. SNAP 398299 is used in research related to anxiety and depression[3-4].
In vitro, HEK-293 cells co-expressing the human Gal3 receptor and Gαz protein were treated with SNAP 398299 (10nM-10μM) for 20 minutes, followed by incubation with 20μM Forskolin for 5 minutes. SNAP 398299 antagonized, in a concentration-dependent manner, the inhibitory effect of galanin on forskolin-stimulated adenylyl cyclase activity[5].
In vivo, rats were treated with an acute intraperitoneal injection of SNAP 398299 (1-10mg/kg) 1-2 hours prior to testing. SNAP 398299 significantly increased active social interaction time in rats, decreased immobility time, and increased swimming time in the forced swim test[5].
References:
[1] Seshan G, Kanagasabai S, Ananthasri S, et al. Insights of structure-based pharmacophore studies and inhibitor design against Gal3 receptor through molecular dynamics simulations. J Biomol Struct Dyn. 2021 Nov;39(18):6987-6999.
[2] Kothandan G, Gadhe CG, Cho SJ. Theoretical characterization of galanin receptor type 3 (Gal3 ) and its interaction with agonist (GALANIN) and antagonists (SNAP 37889 and SNAP 398299): an in silico analysis. Chem Biol Drug Des. 2013 Jun;81(6):757-74.
[3] Ogren SO, Kuteeva E, Hökfelt T, et al. Galanin receptor antagonists : a potential novel pharmacological treatment for mood disorders. CNS Drugs. 2006;20(8):633-54.
[4] Scheller KJ, Williams SJ, Lawrence AJ, et al. An improved method to prepare an injectable microemulsion of the galanin-receptor 3 selective antagonist, SNAP 37889, using Kolliphor(®) HS 15. MethodsX. 2014 Sep 26;1:212-6.
[5] Swanson CJ, Blackburn TP, Zhang X, et al. Anxiolytic- and antidepressant-like profiles of the galanin-3 receptor (Gal3) antagonists SNAP 37889 and SNAP 398299. Proc Natl Acad Sci U S A. 2005 Nov 29;102(48):17489-94.
| Cell experiment [1]: | |
Cell lines | HEK-293 cells (human embryonic kidney cell line) transiently cotransfected with the human Gal3 receptor and Gαz protein |
Preparation Method | HEK-293 cells were treated with SNAP 398299 at concentrations ranging from 10nM to 10μM for 20 minutes. Galanin (1μM) was then added, followed by incubation with 20μM Forskolin for 5 minutes. The inhibition of adenylyl cyclase activity was measured. |
Reaction Conditions | 10nM-10μM; 20min. |
Applications | SNAP 398299 antagonized, in a concentration-dependent and competitive manner, the inhibitory effect of galanin on forskolin-stimulated adenylyl cyclase activity. |
| Animal experiment [1]: | |
Animal models | Adult male Sprague-Dawley rats and infant Hartley guinea pigs |
Preparation Method | Rats received acute oral administration of SNAP 398299 (1, 3, and 10mg/kg) were tested in the forced swim test (FST) 2 hours later. Social interaction tests were conducted 1 hour after oral administration in rats, and guinea pig pups were observed for vocalizations after maternal separation following administration. |
Dosage form | 1, 3, 10mg/kg; p.o.; single administration |
Applications | Acute administration of SNAP 398299 significantly increased active social interaction time in rats, reduced anxiety-related vocalizations in guinea pig pups, and produced antidepressant-like effects in the forced swim test (FST) by decreasing immobility time and increasing swimming time. |
References: | |
| Cas No. | 903878-06-8 | SDF | |
| Canonical SMILES | O=C3/C(C2=CC=CC=C2N3C4=CC(OCCN5CCCC5)=CC=C4)=N/C1=CC=CC(C(F)(F)F)=C1 | ||
| Formula | C27H24F3N3O2 | M.Wt | 479.49 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.0855 mL | 10.4277 mL | 20.8555 mL |
| 5 mM | 417.1 μL | 2.0855 mL | 4.1711 mL |
| 10 mM | 208.6 μL | 1.0428 mL | 2.0855 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 14 reference(s) in Google Scholar.)