GLPBio

SP 600125

Catalog No.: GC15344Purity:>98.00%
COASDSData Sheet
SP 600125 is an orally active, reversible, selective, ATP-competitive JNK inhibitor with IC50 of 40, 40, and 90 nM for JNK1, JNK2, and JNK3, respectively. SP 600125 is commonly used in the research of ovarian cancer, tumors, Parkinson's disease (PD), breast cancer, and asthma.

Products are for research use only. Not for human use. We do not sell to patients.

SP 600125
Cas No.: 129-56-6
10mM (in 1mL DMSO)In Stock
$43.00
1
10mgIn Stock
$36.00
1
50mgIn Stock
$47.00
1
100mgIn Stock
$58.00
1
500mgIn Stock
$350.00
1
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com

Citations

11 publicationsPublications from researchers using this product

Description

SP 600125 is an orally active, reversible, selective, ATP-competitive JNK inhibitor with IC50 of 40, 40, and 90 nM for JNK1, JNK2, and JNK3, respectively. SP 600125 is commonly used in the research of ovarian cancer, tumors, Parkinson's disease (PD), breast cancer, and asthma[1,2].

SP 600125 (10-20 μM; 24 h) reduces PMA-dependent MMP-9 secretion and inhibits c-Jun phosphorylation, and also inhibits PMA-stimulated MMP-9 promoter-driven luciferase reporter gene activity[1].SP 600125 inhibits c-Jun phosphorylation with an IC50 of 5-10 μM. SP 600125(5-10 μM,24h) also inhibits LPS-induced increase COX-2 protein levels and PGE2 production[3].

SP 600125 (10/30/50 mg/kg/day; ip) inhibits MPTP-induced JNK activation and c-Jun phosphorylation in the substantia nigra obturata (SNc) and prevents the decrease in dopamine concentration in the striatum and alleviates behavioral impairment in MPTP-treated mice[2]. SP 600125(30mg/kg/day) can significantly reduce the expression of inflammatory cells in the bronchi and lungs under OVA stimulation[4].

References:
[1]. Shin M, Yan C, Boyd D. An inhibitor of c-jun aminoterminal kinase (SP600125) represses c-Jun activation, DNA-binding and PMA-inducible 92-kDa type IV collagenase expression. Biochim Biophys Acta. 2002 May 8;1589(3):311-6.
[2]. Wang W, Shi L, Xie Y, Ma C, Li W, Su X, Huang S, Chen R, Zhu Z, Mao Z, Han Y, Li M. SP600125, a new JNK inhibitor, protects dopaminergic neurons in the MPTP model of Parkinson's disease. Neurosci Res. 2004 Feb;48(2):195-202.
[3]. Nieminen R, Lahti A, Jalonen U, Kankaanranta H, Moilanen E. JNK inhibitor SP600125 reduces COX-2 expression by attenuating mRNA in activated murine J774 macrophages. Int Immunopharmacol. 2006 Jun;6(6):987-96.
[4].Wu HM, Fang L, Shen QY, Liu RY. SP600125 promotes resolution of allergic airway inflammation via TLR9 in an OVA-induced murine acute asthma model. Mol Immunol. 2015 Oct;67(2 Pt B):311-6.

Protocol

Cell experiment [1]:

Cell lines

 OVCAR-3 cells

Preparation Method

 OVCAR3 cells were treated with SP600125(10-20) μM for 24 hours.

Reaction Conditions

 SP600125(10-20) μM ;24h

Applications

 SP600125(10-20μM;24h) reduces PMA-dependent MMP-9 secretion and inhibits c-Jun phosphorylation.
Animal experiment [2]:

Animal models

 MPTP-induced PD in mice

Preparation Method

 MPTP-induced PD in mice that they were given SP600125 (10/30/50 mg/kg;ip) once per day between days 1 and 6, respectively. Mice were sacrificed at day 10 after the final injection of MPTP.

Dosage form

 SP600125 (10/30/50 mg/kg)/ day; ip

Applications

 SP600125 inhibited MPTP-induced JNK activation and c-Jun phosphorylation in SNc;SP600125 prevented the reduction of dopamine concentrations in the striatum and attenuated behavioral impairment in MPTP-treated mice.

References:
[1]. Shin M, Yan C, Boyd D. An inhibitor of c-jun aminoterminal kinase (SP600125) represses c-Jun activation, DNA-binding and PMA-inducible 92-kDa type IV collagenase expression. Biochim Biophys Acta. 2002 May 8;1589(3):311-6.
[2]. Wang W, Shi L, Xie Y, Ma C, Li W, Su X, Huang S, Chen R, Zhu Z, Mao Z, Han Y, Li M. SP600125, a new JNK inhibitor, protects dopaminergic neurons in the MPTP model of Parkinson's disease. Neurosci Res. 2004 Feb;48(2):195-202.

Product Documents

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Biological Data

1 / 6

Chemical Properties

CAS No.
129-56-6
Chemical Name
dibenzo[cd,g]indazol-6(2H)-one
SMILES
O=C1C2=CC=CC3=C2C(C4=CC=CC=C41)=NN3
Molecular Formula
C14H8N2O
Molecular Weight
220.23
Solubility
≥ 11mg/mL in DMSO, ≥ 2.56 mg/mL in EtOH with gentle warming
Storage
Desiccate at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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