SP 600125 |
Catalog No.GC15344
One-Click Copy Product Info
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SP 600125 is an orally active, reversible, selective, ATP-competitive JNK inhibitor with IC50 of 40, 40, and 90 nM for JNK1, JNK2, and JNK3, respectively. SP 600125 is commonly used in the research of ovarian cancer, tumors, Parkinson's disease (PD), breast cancer, and asthma.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 129-56-6
Sample solution is provided at 25 µL, 10mM.
SP 600125 is an orally active, reversible, selective, ATP-competitive JNK inhibitor with IC50 of 40, 40, and 90 nM for JNK1, JNK2, and JNK3, respectively. SP 600125 is commonly used in the research of ovarian cancer, tumors, Parkinson's disease (PD), breast cancer, and asthma[1,2].
SP 600125 (10-20 μM; 24 h) reduces PMA-dependent MMP-9 secretion and inhibits c-Jun phosphorylation, and also inhibits PMA-stimulated MMP-9 promoter-driven luciferase reporter gene activity[1].SP 600125 inhibits c-Jun phosphorylation with an IC50 of 5-10 μM. SP 600125(5-10 μM,24h) also inhibits LPS-induced increase COX-2 protein levels and PGE2 production[3].
SP 600125 (10/30/50 mg/kg/day; ip) inhibits MPTP-induced JNK activation and c-Jun phosphorylation in the substantia nigra obturata (SNc) and prevents the decrease in dopamine concentration in the striatum and alleviates behavioral impairment in MPTP-treated mice[2]. SP 600125(30mg/kg/day) can significantly reduce the expression of inflammatory cells in the bronchi and lungs under OVA stimulation[4].
References:
[1]. Shin M, Yan C, Boyd D. An inhibitor of c-jun aminoterminal kinase (SP600125) represses c-Jun activation, DNA-binding and PMA-inducible 92-kDa type IV collagenase expression. Biochim Biophys Acta. 2002 May 8;1589(3):311-6.
[2]. Wang W, Shi L, Xie Y, Ma C, Li W, Su X, Huang S, Chen R, Zhu Z, Mao Z, Han Y, Li M. SP600125, a new JNK inhibitor, protects dopaminergic neurons in the MPTP model of Parkinson's disease. Neurosci Res. 2004 Feb;48(2):195-202.
[3]. Nieminen R, Lahti A, Jalonen U, Kankaanranta H, Moilanen E. JNK inhibitor SP600125 reduces COX-2 expression by attenuating mRNA in activated murine J774 macrophages. Int Immunopharmacol. 2006 Jun;6(6):987-96.
[4].Wu HM, Fang L, Shen QY, Liu RY. SP600125 promotes resolution of allergic airway inflammation via TLR9 in an OVA-induced murine acute asthma model. Mol Immunol. 2015 Oct;67(2 Pt B):311-6.
| Cell experiment [1]: | |
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Cell lines |
OVCAR-3 cells |
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Preparation Method |
OVCAR3 cells were treated with SP600125(10-20) μM for 24 hours. |
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Reaction Conditions |
SP600125(10-20) μM ;24h |
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Applications |
SP600125(10-20μM;24h) reduces PMA-dependent MMP-9 secretion and inhibits c-Jun phosphorylation. |
| Animal experiment [2]: | |
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Animal models |
MPTP-induced PD in mice |
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Preparation Method |
MPTP-induced PD in mice that they were given SP600125 (10/30/50 mg/kg;ip) once per day between days 1 and 6, respectively. Mice were sacrificed at day 10 after the final injection of MPTP. |
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Dosage form |
SP600125 (10/30/50 mg/kg)/ day; ip |
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Applications |
SP600125 inhibited MPTP-induced JNK activation and c-Jun phosphorylation in SNc;SP600125 prevented the reduction of dopamine concentrations in the striatum and attenuated behavioral impairment in MPTP-treated mice. |
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References: |
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| Cas No. | 129-56-6 | SDF | |
| Chemical Name | dibenzo[cd,g]indazol-6(2H)-one | ||
| Canonical SMILES | O=C1C2=CC=CC3=C2C(C4=CC=CC=C41)=NN3 | ||
| Formula | C14H8N2O | M.Wt | 220.23 |
| Solubility | ≥ 11mg/mL in DMSO, ≥ 2.56 mg/mL in EtOH with gentle warming | Storage | Desiccate at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.5407 mL | 22.7035 mL | 45.4071 mL |
| 5 mM | 908.1 μL | 4.5407 mL | 9.0814 mL |
| 10 mM | 454.1 μL | 2.2704 mL | 4.5407 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Related Biological Data
SP mediates renal inflammation and fibrosis through an NK-1R-dependent JNK/p38 mechanism in vivo and in vitro.(I) p38- or JNK-specific inhibitors or their combination significantly attenuated SP-stimulated G2/M arrest and apoptosis.
NK-1R-overexpressed HK-2 cells were incubated with the indicated treatment (20 mM SP, 10 mM SR140333, 5 mM SP600125 (GlpBio), 2.5 mM SB239063) for 48 hours, and then evaluated by staining with Annexin V/DAPI staining.
Front Immunol 14 (2023): 1142240. PMID: 37033943 IF: 8.7864 -
Related Biological Data
P38/JNK is involved in CaM-mediated apoptosis under AngII stimulation. c–f The protein expression levels of Bcl-2 and Bax in each group were detected and quantitatively analyzed.
The results showed that treatment with SP600125 (10 μM)(GlpBio) and SB203580 (10 μM) led to a decrease in Bax expression and an increase in Bcl-2 expression.
Acta Pharmacol Sin (2023): 1-15. PMID: 37833535 IF: 8.1996 -
Related Biological Data
tBHQ induces the Nrf2-dependent antioxidant responses in glutamate-induced R28 cells B. Effects of pretreatment with inhibitors of intermediate proteins on cell viability.
Since activation of intermediate proteins such as ERK, JNK, p38 or Akt is associated with Nrf2 translocation, we pretreated cells for 30 min with inhibitors of ERK (PD98059), JNK (SP600125)(GlpBio), p38 (SB203580) or Akt (MK-2206) to determine if tBHQ affects these intermediate proteins
Biomed Pharmacother 152 (2022): 113117. PMID: 35653886 IF: 6.5298 -
Related Biological Data
PFN1-Cdc42 negatively regulates bovine skeletal muscle via PAK/JNK signaling pathway: (D) protein level analysis of P-JNK after P-PAK inhibition in DM2 bovine myoblasts by Western blot;
The secondary antibodies included horseradish peroxidase goat anti-mouse/rabbit IgG (1:10000), PAK inhibitors PF-3758309,and JNK inhibitors (GLPBIO, SP600125).
Cells 11.20 (2022): 3188. PMID: 36291059 IF: 6 -
Related Biological Data
Effects of 5-HT and 5-HT7R selective agonist LP-12 combined with SB269970 on melanin contents, dendrite, or migration in B16F10 cells.(C)B16F10 cells were pre-incubated with SP600125 (10μM) and PD98059 (10μM) for 2h prior to the addition of LP-12 (200nM), and then incubated for 72h for the measurement of melanin content.
B16F10 cells were pre-incubated with SP600125 (10μM)(GlpBio) and PD98059 (10μM) for 2h prior to the addition of LP-12 (200nM), and then incubated for 72h for the measurement of melanin content.
Faseb J 37.4 (2023): e22893. PMID: 36961387 IF: 5.8339 -
Related Biological Data
Effect of KLF5 on the JNK signaling pathway in ATC cells.C and D, The protein expression of P‐gp and ABCG2 was measured by Western blot analysis in Hth74 and Hth74Rdox cells treated with 20 μM SP600125 for 24 hours.
The protein expression of P‐gp and ABCG2 was measured by Western blot analysis in Hth74 and Hth74Rdox cells treated with 20 μM SP600125(GlpBio) for 24 hours.
J Biochem Mol Toxic 34.5 (2020): e22469. PMID: 32173973 IF: 3.643
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