Triflumuron (Synonyms: OMS-2015) |
Catalog No.GC48203
One-Click Copy Product Info
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Triflumuron is a synthetic insecticide from the active ingredient group of chitin biosynthesis inhibitors with IC50 value of 1.48 ± 1.44μM, and with EI50 values of 5.28 and 1.59μg/L against Culex quinquefasciatus and Aedes albopictus, respectively.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 64628-44-0
Sample solution is provided at 25 µL, 10mM.
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Triflumuron is a synthetic insecticide from the active ingredient group of chitin biosynthesis inhibitors with IC50 value of 1.48 ± 1.44μM, and with EI50 values of 5.28 and 1.59μg/L against Culex quinquefasciatus and Aedes albopictus, respectively. Triflumuron interferes with chitin synthesis and the moulting cycle, disrupting chitin deposition in the insect cuticle after ingestion[1][2][3].
In vitro, Triflumuron(1μM to 1mM) was tested for its ability to inhibit hyaluronan (HA) secretion in NIH3T3 fibroblast cells. The results showed that Triflumuron effectively reduced HA deposition in the cell culture media, with a half-maximal inhibitory concentration (IC50) of 1.48 ± 1.44μM[1]. Triflumuron (50‐300μM) treated human renal embryonic cells (HEK 293) and hepatocytes (Hep G2) reduced significantly the cell viability and increased the reactive oxygen species generation, malondialdehyde levels, and mitochondrial membrane potential in both cell lines. Triflumuron was also demonstrated as an inductor of DNA damages quantified by the comet assay[4].
In vivo, single intraperitoneal injection of Triflumuron (250, 350, 500mg/kg) in Balb/C mice induced oxidatie stress and elevated protein carbonyl levels in both liver and kidney extracts. The results also showed a significant increase in superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase (GST) activity in liver and kidney after Triflumuron administration[5]. Triflumuron (250, 350, 500mg/kg) was administered intraperitoneally to Balb/C male mice for 24h. The results indicated that Triflumuron has genotoxic effects on mice bone marrow cells, causing DNA damage, micronuclei formation, and chromosome aberrations in a dose-dependent manner[6].
References:
[1] Tsitrina A A, Krasylov I G, Maltsev D I, et al. Inhibition of hyaluronan secretion by novel coumarin compounds and chitin synthesis inhibitors. Glycobiology. 2021 Sep 9;31(8):959-974.
[2] Belinato T A, Martins A J, Lima J B P, Valle D. Effect of triflumuron, a chitin synthesis inhibitor, on Aedes aegypti, Aedes albopictus and Culex quinquefasciatus under laboratory conditions. Parasit Vectors. 2013 Apr 4:6:83.
[3] Batra C P, Mittal P K, Adak T, Ansari M A. Efficacy of IGR compound Starycide 480 SC (Triflumuron) against mosquito larvae in clear and polluted water. J Vector Borne Dis.2005 Sep;42(3):109-16.
[4] Timoumi R, Amara I, Salem I B, Abid-Essefi S. Triflumuron induces cytotoxic effects on hepatic and renal human cell lines. J Biochem Mol Toxicol. 2020 Aug;34(8):e22504.
[5] Timoumi R, Amara I, Neffati F, et al. Acute triflumuron exposure induces oxidative stress responses in liver and kidney of Balb/C mice. Environ Sci Pollut Res Int. 2019 Feb;26(4):3723-3730.
[6] Timoumi R, Amara I, Ayed Y. et al. Triflumuron induces genotoxicity in both mice bone marrow cells and human Colon cancer cell line. Toxicol Mech Methods. 2020 Jul;30(6):438-449.
| Cell experiment [1]: | |
Cell lines | Hep G2 and HEK 293 cells |
Preparation Method | We used 96‐well plates to seed and incubate (2.5 × 105 cells) for 24 hours with different concentrations of Triflumuron (10-500μM) before cells were used to perform MTT assay. Triflumuron concentration range (50‐300μM) was used to treat Hep G2 and HEK 293 cells before ROS generation, antioxidant enzymes acitvities, total score of DNA damage and protein expression level were also evaluated. |
Reaction Conditions | 10 to 500µM or 50 to 300µM; 24h |
Applications | Treatment of cells with increasing concentrations of Triflumuron decreased significantly the cell viability. Triflumuron is an oxidizing agent in both renal and hepatic cells by increasing ROS production, which alters the normal functioning of antioxidant enzymes such as CAT and SOD, which consequentlydamage cells by oxidizing lipids, disrupting DNA and proteins. |
| Animal experiment [2]: | |
Animal models | Male Balb/C mice |
Preparation Method | After acclimation, mice were randomly divided into four groups of six each as follows: Group 1: mice given the vehicle, ethanol/water (1:1, v:v) Groups 2, 3, and 4: mice given Triflumuron at 250, 350, and 500mg/kg bw corresponding respectively to 5, 7, and 10% of the LD50. A single administration by intra-peritoneal injection of 100μl of solutions was performed. The sacrifice was done 24h after injections by cervical dislocation. |
Dosage form | 250, 350, and 500mg/kg; i.p.; a single administration |
Applications | In treated mice, the levels of malondialdehyde (MDA), an ultimate fragment of the degradation of cell membrane polyunsaturated fatty acids, significantly increased in the liver, while only the highest dose induced lipid peroxidation in kidney tissues. Triflumuron treatment also caused protein damage via free radicals. As an adaptive response, antioxidant enzyme activities notably rose in both organs. |
References: | |
| Cas No. | 64628-44-0 | SDF | |
| Synonyms | OMS-2015 | ||
| Canonical SMILES | ClC1=CC=CC=C1C(NC(NC2=CC=C(OC(F)(F)F)C=C2)=O)=O | ||
| Formula | C15H10ClF3N2O3 | M.Wt | 358.7 |
| Solubility | DMF: 30 mg/ml,DMSO: 10 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7878 mL | 13.9392 mL | 27.8784 mL |
| 5 mM | 557.6 μL | 2.7878 mL | 5.5757 mL |
| 10 mM | 278.8 μL | 1.3939 mL | 2.7878 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)