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Ultra High Sensitivity ECL Kit

Catalog No.GK10008

Luminol-based chemiluminescent kit for detection of horseradish peroxidase (HRP)-labeled antibodies and their associated antigens.

Products are for research use only. Not for human use. We do not sell to patients.

Ultra High Sensitivity ECL Kit Chemical Structure

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Sample solution is provided at 25 µL, 10mM.

Product has been cited by 73 publications

Description Protocol Components Features & Properties Quality Control Product Documents Related Video

The core principle of ECL reagent detection is the luminescence of the oxidation reaction: Luminol (lumino) is the main component of the luminescence substrate. Under alkaline conditions, it is catalyzed by H2O2 to generate 3-amino ozone. The excited state intermediate of phthalic acid emits photons when it returns to the ground state. The maximum emission wavelength is 425 nm. The photon signal can be captured by X-ray film or CCD imager.

The GlpBio Ultra High Sensitivity ECL kit can oxidize luminol in the presence of HRP and peroxide to detect within the femtogram range of antigens. This reaction produces prolonged chemiluminescence, which can be seen on X-ray film or digital imaging systems. The GlpBio Ultra High Sensitivity ECL kit can produce a powerful, long-lived signal. Coupled with a very low background level, it can extend the exposure time to detect low-abundance proteins.Principle of ECL kit


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Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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