DAF-2 (Synonyms: 4,5-Diaminofluorescein)

This plan only provides a guide, please modify it to meet your specific needs.

1. This product is provided in the form of DMSO stock solution with a concentration of 1mM. It is not recommended to change the solvent;

2. It is recommended that unused stock solutions be aliquoted and stored in the dark at -20°C or -80°C to avoid repeated freezing and thawing;

3．Please centrifuge briefly before using the dye to ensure that the product is concentrated at the bottom of the tube;

4. BSA and phenol red interfere with the detection of DAF-2. It is recommended to use a suitable buffer (such as serum- and phenol red-free medium or PBS) to dilute the stock solution and prepare a working solution with a concentration of 1-10μM;

5. The diluted water-soluble solution should not be stored for more than one day. Please adjust the concentration of the working solution according to the actual situation and make it ready for use;

6．Microscope detection: The maximum excitation/emission wavelength of DAF-2 is 485/538nm.

7. DAF-2 is not cell membrane permeable and can be used for direct detection of nitric oxide in extracellular or solution systems. If you need to detect intracellular nitric oxide, you can choose DAF-2 DA (GC30702), a cell membrane-permeable DAF-2 derivative;

8. Fluorescent dyes all have quenching problems. Please avoid light as much as possible to slow down fluorescence quenching.

9. For your safety and health, please wear a lab coat and disposable gloves.